Molecular beacon-based quantitiation of epithelial tumor marker mucin 1
| Autor: | Seonmi Shin, Woong Jung, Hye Yeon Nam, Sang Soo Hah, Eun Jeong Lee |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Aptamer
Clinical Biochemistry Quantitative proteomics Pharmaceutical Science Biochemistry Molecular beacon Quantum Dots Drug Discovery Biomarkers Tumor Fluorescence Resonance Energy Transfer Humans Neoplasms Glandular and Epithelial Molecular Biology MUC1 Tumor marker chemistry.chemical_classification Chemistry Mucin-1 Organic Chemistry Mucin Aptamers Nucleotide Carbocyanines Molecular biology Förster resonance energy transfer Molecular Probes Molecular Medicine Glycoprotein |
| Zdroj: | Bioorganic & Medicinal Chemistry Letters. 22:6081-6084 |
| ISSN: | 0960-894X |
| DOI: | 10.1016/j.bmcl.2012.08.037 |
| Popis: | Mucin 1 (Muc1) is a glycoprotein expressed on most epithelial cell surfaces, which has been confirmed as a useful biomarker for the diagnosis of early cancers. In this study, we demonstrate that a quantum dot (QD)-aptamer beacon acts by folding-induced dissociation of a DNA intercalating dye, BOBO-3, in the presence of the target molecules, Muc1. Release of intercalated BOBO-3s from the QD-conjugated aptamers results in a decrease in QD fluorescence resonance energy transfer (FRET)-mediated BOBO-3 emission, allowing for label-free Muc1 detection and quantitation. We attain highly specific and wide-range detection (from 50nM to 20μM) of Muc1, suggesting that our QD-aptamer beacon can be a potential alternative to immuno-based assays for Muc1 detection. The detection methodology is expected to be improved for the early diagnosis of different types of epithelial cancers of large populations. |
| Databáze: | OpenAIRE |
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