Polaromonas ginsengisoli sp. nov., isolated from ginseng field soil
| Autor: | Muhammad Zubair Siddiqi, Qing-Mei Liu, Se Yoon Chun, Soon Youl Lee, Jung Hun Jo, Gyu-Min Choi, Sang Young Kim, Wan-Taek Im, Kang Duk Choi |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
DNA Bacterial Ubiquinone Panax Polaromonas vacuolata Microbiology Agar plate Comamonadaceae 03 medical and health sciences Ginseng RNA Ribosomal 16S Republic of Korea Ecology Evolution Behavior and Systematics Polaromonas Phospholipids Phylogeny Soil Microbiology Base Composition biology Phylogenetic tree Strain (chemistry) Fatty Acids Nucleic Acid Hybridization General Medicine Sequence Analysis DNA 16S ribosomal RNA biology.organism_classification Bacterial Typing Techniques 030104 developmental biology Bacteria |
| Zdroj: | International journal of systematic and evolutionary microbiology. 68(5) |
| ISSN: | 1466-5034 |
| Popis: | A Gram-reaction-negative, strictly aerobic, milky-white and rod-shaped bacterium (designated Gsoil 115T) isolated from ginseng field soil was characterized by a polyphasic approach to clarify its taxonomic position. Strain Gsoil 115T grew optimally at 30 °C and at pH 7.0 on Reasoner’s 2A agar medium. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Gsoil 115T belongs to the genus Polaromonas and was most closely related to Polaromonas eurypsychrophila B717-2T (98.6 %), Polaromonas vacuolata 34-PT (98.3 %), Polaromonas jejuensis NBRC 106434T (98.1 %), Polaromonas aquatic CCUG 39402T (97.7 %) and Polaromonas cryoconiti Cr4-35T (97.5 %). The DNA G+C content was 60.9 mol%. The DNA–DNA hybridization relatedness between strain Gsoil 115T and P. eurypsychrophila B717-2T, P. vacuolata 34-PT, P. jejuensis NBRC 106434T, P. aquatic CCUG 39402T and P. cryoconiti Cr4-35T were 31.2, 21.6, 16.9, 8.7 and 10.1 %, respectively. The major polar lipids were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG) and phosphatidylethanolamine (PE). The sole respiratory quinone was Q-8. The major fatty acids were C16 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1 ω6c), which supported the affiliation of strain Gsoil 115T to the genus Polaromonas . Moreover, the physiological, biochemical and low level of DNA–DNA relatedness value allowed the phenotypic and genotypic differentiation of strain Gsoil 115T from the recognized species of the genus Polaromonas . Therefore, strain Gsoil 115T represents a novel species of the genus Polaromonas , for which the name Polaromonas ginsengisoli sp. nov. is proposed, with the type strain Gsoil 115T (LMG 23393T=KCTC 12577T). |
| Databáze: | OpenAIRE |
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