Modified Biosensor for Cholinesterase Inhibitors with Guinea Green B as the Color Indicator
| Autor: | Martin Lobotka, Jan Dědič, Lukáš Matějovský, Vladimír Pitschmann, Martin Urban, Michal Dymák |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Sarin
lcsh:Biotechnology Clinical Biochemistry Cyclosarin 02 engineering and technology macromolecular substances Biosensing Techniques 01 natural sciences Article chemistry.chemical_compound Lissamine Green Dyes lcsh:TP248.13-248.65 Soman enzymatic reaction Detection limit Chromatography Chromogenic visual evaluation 010401 analytical chemistry technology industry and agriculture General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry chemical warfare agents Reagent Colorimetry Naked eye Cholinesterase Inhibitors Guinea Green B 0210 nano-technology Biosensor biosensor of cholinesterase inhibitors |
| Zdroj: | Biosensors Volume 8 Issue 3 Biosensors, Vol 8, Iss 3, p 81 (2018) |
| ISSN: | 2079-6374 |
| Popis: | Colorimetric biosensors of cholinesterase inhibitors are ideal for fast, reliable, and very simple detection of agents in air, in water, and on surfaces. This paper describes an innovation of the Czech Detehit biosensor, which is based on a biochemical enzymatic reaction visualized by using Ellman&rsquo s reagent as a chromogenic indicator. The modification basically consists of a much more distinct color response of the biosensor, attained through optimization of the reaction system by using Guinea Green B as the indicator. The performance of the modified biosensor was verified on the chemical warfare agents (sarin, soman, cyclosarin, and VX) in water. The detection limits ascertained visually (with the naked eye) were about 0.001 µ g/mL in water (exposure time 60 s, inhibition efficiency 25%). |
| Databáze: | OpenAIRE |
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