Characterization of a cell type-specific enhancer found in the human papilloma virus type 18 genome
| Autor: | F V Swift, H Hamada, H B Younghusband, K Bhat |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Genes
Viral Enhancer RNAs Biology Genome General Biochemistry Genetics and Molecular Biology Transcription (biology) Genes Regulator Humans Enhancer trap Cloning Molecular Enhancer Papillomaviridae Molecular Biology Gene Southern blot Genetics Base Sequence General Immunology and Microbiology General Neuroscience Adenovirus Early Proteins Nucleic acid sequence DNA Restriction Enzymes Oncogene Proteins Viral Molecular biology Enhancer Elements Genetic Genes Mutation Chromosome Deletion Research Article HeLa Cells |
| Zdroj: | The EMBO Journal. 6:1339-1344 |
| ISSN: | 0261-4189 |
| DOI: | 10.1002/j.1460-2075.1987.tb02373.x |
| Popis: | We have previously isolated long-range acting enhancer elements from the HeLa genome by functional selection. In this report, the structural and functional characteristics of one (GA1) of the enhancers are reported. By cloning various restriction fragments and by deletion mutagenesis, the activity of GA1 was located in a 230-bp region. The nucleotide sequence of GA1 and genomic Southern blot analysis indicated that GA1 is derived from human papilloma virus (HPV) 18 DNA that had been integrated into the HeLa genome. The enhancer is located in the non-coding region of the HPV 18 genome. The HPV 18 enhancer consists of two functional domains, both of which have full enhancer activity in HeLa cells. The enhancer does not contain enhancer core sequences but contains several blocks of potential Z-DNA sequence. Like SV40 and polyoma virus enhancers, the activity of the HPV 18 enhancer was repressed by adenovirus E1a products. The HPV 18 enhancer shows a narrow cell type specificity: it is active in some cervical carcinoma cell lines, but inactive in all non-cervical cells except for one neuroblastoma cell line. These results suggest that the HPV 18 enhancer plays an important role in regulation of the viral genes. |
| Databáze: | OpenAIRE |
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