Phosphoric acid concentration affects dentinal MMPs activity
| Autor: | A.G. Devito-Moraes, Polliana Mendes Candia Scaffa, Fabio D. Nascimento, D. Nesadal, Carlos Eduardo Francci, L.C. Yamasaki, Jose C. Nicolau, M.R. Carrilho, D.H. Pashley, Cristina de Mattos Pimenta Vidal |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Materials science
Gelatin Zymography chemistry.chemical_element 02 engineering and technology Calcium Matrix metalloproteinase 03 medical and health sciences Hydroxyproline chemistry.chemical_compound 0302 clinical medicine stomatognathic system Dentin medicine Humans Phosphoric Acids General Materials Science Tooth Demineralization General Dentistry Phosphoric acid chemistry.chemical_classification Chromatography 030206 dentistry 021001 nanoscience & nanotechnology Matrix Metalloproteinases Demineralization stomatognathic diseases medicine.anatomical_structure Enzyme Biochemistry chemistry Mechanics of Materials 0210 nano-technology Nuclear chemistry |
| Zdroj: | Dental Materials. 34:e79 |
| ISSN: | 0109-5641 |
| DOI: | 10.1016/j.dental.2018.08.164 |
| Popis: | To evaluate whether the concentration of phosphoric acid (PA) has an effect on the proteolytic activity of sound human demineralized dentin. It is hypothesized that the activity of matrix-bound and extracted enzymes depends on the PA concentration used to demineralize dentin.One-gram aliquots of mid-coronal human dentin powder were demineralized with 1wt%, 10wt% and 37wt% PA. Concentrations of released calcium were measured for each set of demineralization. Extracted MMP-2 was immunologically identified by western blot and its activity was determined by conventional gelatin zymography. Analysis of released hydroxyproline (HYP) and in situ zymography were performed to evaluate the activity of insoluble, bound-matrix enzymes.The amount of released calcium from dentin powder treated with 37wt% PA was significantly higher (p≤0.05) than that obtained by dentin demineralization with 10wt% and 1wt% PA. Expression and activity of endogenous enzymes, extracted from or bound to dentin matrix, were detected for all samples regardless of the PA concentration. However, the expression and activity of extracted MMP-2 were significantly higher when dentin was treated with 10wt% PA (p0.05), followed by 1wt% and 37wt% PA. Similarly, the highest concentration of released HYP (i.e. meaning higher percentage of collagen degradation) and the highest activity in in situ zymography were observed when dentin samples were treated with 10wt% PA (p0.05).It was confirmed that PA does not denature endogenous enzymes of dentin matrices, but it may somehow modulate the expression and activity of these enzymes in a concentration-dependent manner.Endogenous proteases have been identified and suggested to be responsible for the digestion of dentin matrix when activated by the acidic components of dental adhesives. Proteolytic activity of dentinal MMPs showed to be dependent on phosphoric acid concentration. The clinically-used concentration (37%) does not inhibit MMPs activity, but slows it. |
| Databáze: | OpenAIRE |
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