Antagonistic bioaccumulation of waterborne Cu(II) and Cd(II) in common carp (Cyprinus carpio) and effects on ion-homeostasis and defensive mechanisms

Autor: Lieven Bervoets, G. Flipkens, G. Castaldo, M. Pillet, Raewyn M. Town, G. De Boeck, R. Blust
Přispěvatelé: Systemic Physiological and Ecotoxicological Research, Department of Biology, University of Antwerp
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Gills
Carps
Health
Toxicology and Mutagenesis
Metal ions in aqueous solution
Population
010501 environmental sciences
Aquatic Science
01 natural sciences
Cyprinus
Lethal Dose 50
Metal
Electrolytes
03 medical and health sciences
Common carp
Animals
Homeostasis
education
Biology
ComputingMilieux_MISCELLANEOUS
Copper Transporter 1
030304 developmental biology
0105 earth and related environmental sciences
0303 health sciences
education.field_of_study
Ion Transport
biology
Chemistry
Pharmacology. Therapy
Sodium
biology.organism_classification
Bioaccumulation
Ion homeostasis
Biochemistry
visual_art
Toxicity
Potassium
visual_art.visual_art_medium
[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology
Sodium-Potassium-Exchanging ATPase
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
Copper
Water Pollutants
Chemical
Cadmium
Zdroj: Aquatic Toxicology
Aquatic Toxicology, Elsevier, 2020, pp.105561. ⟨10.1016/j.aquatox.2020.105561⟩
Aquatic toxicology
ISSN: 0166-445X
DOI: 10.1016/j.aquatox.2020.105561⟩
Popis: In the aquatic environment, metals are present as mixtures, therefore studies on mixture toxicity are crucial to thoroughly understand their toxic effects on aquatic organisms. Common carp were used to assess the effects of short-term Cu and Cd mixtures, using a fixed concentration representing 25% of the 96h-LC50 (concentration lethal for 50% of the population) of one of the metals combined with a variable concentration of 10, 25 or 50% of the 96h-LC50 of the other metal, and vice versa. Our results showed a fast Cu and Cd accumulation, with the percentage increase in the order gill > liver > carcass. An inhibitory effect of Cu on Cd uptake was observed; higher Cu concentrations at fixed Cd levels resulted in a decreased accumulation of Cd. The presence of the two metals resulted in Na, K and Ca losses. The disturbance in the ion-homeostasis was sensed by the organisms, and they tried to compensate for the Na loss through the induction of the genes coding for Na+/K+-ATPase and H+-ATPase. In contrast to our expectation, a decreased gene expression of NHE was observed. Additionally, a counterintuitive induction of the CTR1 gene occurred, as a downregulation was expected to prevent further metal uptake. An induction of defensive mechanisms, both metal binding protein and anti-oxidant defences, was observed. Despite the metal accumulation and electrolyte loss, the low mortality suggest that common carp is able to cope with these metal levels, at least during a one-week exposure.
Databáze: OpenAIRE
Externí odkaz: