Engineering Biomimetic Trogocytosis with Farnesylated Chemically Self-Assembled Nanorings
| Autor: | Yiao Wang, Lakmal Rozumalski, Ozgun Kilic, Caitlin Lichtenfels, Jacob Petersberg, Mark D. Distefano, Carston R. Wagner |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Biomacromolecules |
| ISSN: | 1526-4602 1525-7797 |
| DOI: | 10.1021/acs.biomac.2c00837 |
| Popis: | Inspired by the natural intercellular material transfer process of trans-endocytosis or trogocytosis, we proposed that targeted farnesylated chemically self-assembled nanorings (f-CSANs) could serve as a biomimetic trogocytosis vehicle for engineering directional cargo transfer between cells; thus, allowing cell-cell interactions to be monitored and facilitating cell-cell communications. The membranes of sender cells were stably modified by hydrophobic insertion with the targeted f-CSANs, which were efficiently transferred to receiver cells expressing the appropriate receptors by endocytosis. CSAN-assisted cell-cell cargo transfer (C4T) was demonstrated to be receptor-specific, and dependent on direct cell-cell interactions, the rate of receptor internalization and the level of receptor expression. In addition, C4T was shown to facilitate cell-to-cell delivery of an apoptosis inducing drug, as wells as antisense oligonucleotides (ASO). Taken together, the C4T approach is a potentially versatile biomimetic trogocytosis platform that can be deployed as a macro-chemical biological tool for monitoring cell-cell interactions and engineering cell-cell communications. |
| Databáze: | OpenAIRE |
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