Vitamin C down-regulates VEGF production in B16F10 murine melanoma cells via the suppression of p42/44 MAPK activation
Autor: | Wang Jae Lee, Byung Joo Cho, Joo Myung Kong, Seyeon Bae, Hang Rae Kim, Yong Sung Kim, Jae Seung Kang, Ha Na Kim, Naeun Lee, Young-il Hwang, Seung Koo Lee, Hyemin Kim |
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Rok vydání: | 2011 |
Předmět: |
Vascular Endothelial Growth Factor A
Vitamin MAPK/ERK pathway medicine.medical_specialty Melanoma Experimental Down-Regulation Ascorbic Acid Biochemistry Dinoprostone Mice chemistry.chemical_compound Mediator Cell Line Tumor Internal medicine medicine Animals Phosphorylation Molecular Biology Nitrobenzenes Mitogen-Activated Protein Kinase 1 Sulfonamides Mitogen-Activated Protein Kinase 3 Cyclooxygenase 2 Inhibitors Chemistry Kinase Cell Biology Ascorbic acid Enzyme Activation Vascular endothelial growth factor Vascular endothelial growth factor A Endocrinology Cyclooxygenase 2 Cancer research Signal transduction |
Zdroj: | Journal of Cellular Biochemistry. 112:894-901 |
ISSN: | 0730-2312 |
Popis: | It is known that vitamin C induces apoptosis in several kinds of tumor cells, but its effect on the regulation of the angiogenic process of tumors is not completely studied. Vascular endothelial growth factor (VEGF) is the most well-known angiogenic factor, and it has a potent function as a stimulator of endothelial survival, migration, as well as vascular permeability. Therefore, we have investigated whether vitamin C can regulate the angiogenic process through the modulation of VEGF production from B16F10 melanoma cells. VEGF mRNA expression and VEGF production at protein levels were suppressed by vitamin C. In addition, we found that vitamin C suppressed the expression of cyclooxygenase (COX)-2 and that decreased VEGF production by vitamin C was also restored by the administration of prostaglandin E2 which is a product of COX-2. These results suggest that vitamin C suppresses VEGF expression via the regulation of COX-2 expression. Mitogen-activated protein kinases are generally known as key mediators in the signaling pathway for VEGF production. In the presence of vitamin C, the activation of p42/44 MAPK was completely inhibited. Taken together, our data suggest that vitamin C can down-regulate VEGF production via the modulation of COX-2 expression and that p42/44 MAPK acts as an important signaling mediator in this process. |
Databáze: | OpenAIRE |
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