A simplified minimal residual disease polymerase chain reaction method at early treatment points can stratify children with acute lymphoblastic leukemia into good and poor outcome groups
| Autor: | Mônica Aparecida Ganazza, Silvia R. Toledo, Carlos Alberto Scrideli, Luiz Gonzaga Tone, Marcos Borato Viana, Maria Lúcia M. Lee, Elisabete Delbuono, José Andrés Yunes, Juliana Godoy Assumpção, Rosane Gomes de Paula Queiroz, Antonio Sergio Petrilli, Silvia Regina Brandalise, Marcela de Araújo, Andrea Biondi |
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| Přispěvatelé: | Scrideli, C, Assumpção, J, Ganazza, M, Araújo, M, Toledo, S, Lee, M, Delbuono, E, Petrilli, A, Queiróz, R, Biondi, A, Viana, M, Yunes, J, Brandalise, S, Tone, L |
| Rok vydání: | 2009 |
| Předmět: |
Male
medicine.medical_specialty Neoplasm Residual Adolescent Receptors Antigen T-Cell Kaplan-Meier Estimate Acute lymphoblastic leukemia Gastroenterology Polymerase Chain Reaction Sensitivity and Specificity Immunoglobulin kappa-Chains Antigens CD Internal medicine Acute lymphocytic leukemia Antineoplastic Combined Chemotherapy Protocols medicine Humans Child Childhood Acute Lymphoblastic Leukemia Survival rate Proportional Hazards Models Gene Rearrangement Hematology IG business.industry Minimal residual disease Infant Reproducibility of Results Gene rearrangement Precursor Cell Lymphoblastic Leukemia-Lymphoma medicine.disease Flow Cytometry Prognosis Childhood medicine.anatomical_structure Real-time polymerase chain reaction Treatment Outcome Child Preschool Immunology Multivariate Analysis Female Original Article Bone marrow business Immunoglobulin Heavy Chains TCR |
| Zdroj: | Haematologica. 94(6) |
| ISSN: | 1592-8721 |
| Popis: | Background: Minimal residual disease is an important independent prognostic factor in childhood acute lymphoblastic leukemia. The classical detection methods such as multiparameter flow cytometry and real-time quantitative polymerase chain reaction analysis are expensive, time-consuming and complex, and require considerable technical expertise. Design and Methods: We analyzed 229 consecutive children with acute lymphoblastic leukemia treated according to the GBTLI-99 protocol at three different Brazilian centers. Minimal residual disease was analyzed in bone marrow samples at diagnosis and on days 14 and 28 by conventional homo/heteroduplex polymerase chain reaction using a simplified approach with consensus primers for IG and TCR gene rearrangements. Results: At least one marker was detected by polymerase chain reaction in 96.4% of the patients. By combining the minimal residual disease results obtained on days 14 and 28, three different prognostic groups were identified: minimal residual disease negative on days 14 and 28, positive on day 14/negative on day 28, and positive on both. Five-year event-free survival rates were 85%, 75.6%, and 27.8%, respectively (p |
| Databáze: | OpenAIRE |
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