The Silencing of a 14-3-3ɛ Homolog in Tenebrio molitor Leads to Increased Antimicrobial Activity in Hemocyte and Reduces Larval Survivability
| Autor: | Yongseok Lee, Gi Won Seo, Yong Hun Jo, Hamisi Tindwa, Ki Beom Park, Yeon Soo Han, Sun-Am Kim, Jeong Hwan Seong, Bharat Bhusan Patnaik, Yu Jung Kim |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
lcsh:QH426-470 Antimicrobial peptides Tenebrio molitor Tm14-3-3 epsilon AMP secretion RNA interference innate immunity Peptide binding Biology medicine.disease_cause Tm14-3-3ɛ Article 03 medical and health sciences Hemolymph Genetics medicine Secretion Nuclear export signal Escherichia coli Genetics (clinical) Innate immune system Cell biology lcsh:Genetics 030104 developmental biology Biochemistry |
| Zdroj: | Genes, Vol 7, Iss 8, p 53 (2016) Genes Genes; Volume 7; Issue 8; Pages: 53 GENES(7): 8 |
| ISSN: | 2073-4425 |
| Popis: | The 14-3-3 family of phosphorylated serine-binding proteins acts as signaling molecules in biological processes such as metabolism, division, differentiation, autophagy, and apoptosis. Herein, we report the requirement of 14-3-3 epsilon isoform from Tenebrio molitor (Tm14-3-3 epsilon) in the hemocyte antimicrobial activity. The Tm14-3-3 epsilon transcript is 771 nucleotides in length and encodes a polypeptide of 256 amino acid residues. The protein has the typical 14-3-3 domain, the nuclear export signal (NES) sequence, and the peptide binding residues. The Tm14-3-3 epsilon transcript shows a significant three-fold expression in the hemocyte of T. molitor larvae when infected with Escherichia coli Tm14-3-3 epsilon silenced larvae show significantly lower survival rates when infected with E. coli. Under Tm14-3-3 epsilon silenced condition, a strong antimicrobial activity is elicited in the hemocyte of the host inoculated with E. coli. This suggests impaired secretion of antimicrobial peptides (AMP) into the hemolymph. Furthermore, a reduction in AMP secretion under Tm14-3-3 epsilon silenced condition would be responsible for loss in the capacity to kill bacteria and might explain the reduced survivability of the larvae upon E. coli challenge. This shows that Tm14-3-3 epsilon is required to maintain innate immunity in T. molitor by enabling antimicrobial secretion into the hemolymph and explains the functional specialization of the isoform. |
| Databáze: | OpenAIRE |
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