Tin Selenide Quantum Dots Electrochemical Biotransducer for the Determination of Indinavir - A Protease Inhibitor Anti-Retroviral Drug
| Autor: | Rachel Fanelwa Ajayi, Emmanuel I. Iwuoha, Samantha F. Douman, Abongile N. Jijana, Usisipho Feleni, Unathi Sidwaba, Priscilla G. L. Baker |
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| Rok vydání: | 2016 |
| Předmět: |
Detection limit
Band gap Chemistry Tin selenide Analytical chemistry 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences chemistry.chemical_compound Quantum dot Indinavir medicine Biotransducer Solubility 0210 nano-technology Biosensor Nuclear chemistry medicine.drug |
| Zdroj: | Journal of Nano Research. 44:196-207 |
| ISSN: | 1661-9897 |
| Popis: | Biocompatibility of tin selenide quantum dots was achieved by the incorporation of 3-mercaptopropionic acid (3-MPA) as a capping agent, which also improved the stability and the solubility of the material. The UV-Vis spectrophotometric analysis of the quantum dots revealed a broad absorption band at ~ 330 nm (with a corresponding band gap, Eg, value of 3.75 eV), which is within the range of values expected for quantum dots materials. The 3-mercaptopropionic acid-capped tin selenide (3-MPA-SnSe) quantum dots were used to develop an electrochemical biosensor for indinavir, which is a protease inhibitor antiretroviral (ARV) drug. The biosensor was prepared by the self-assembly of L-cysteine on a gold electrode that was functionalised with 3-MPA-SnSe quantum dots, followed by cross-linking with cytochrome P450-3A4 (CYP3A4) using 1-ethyl-3(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). The electrocatalytic properties of the biosensor included a characteristic cyclic voltammetric reduction peak at-380 mV, which was used to detect the response of the biosensor to indinavir. The sensor performance parameters included response time and limit of detection (LOD) values of 11 s and 3.22 pg/mL, respectively. The test concentration range studied (0.014 – 0.066 ng/mL) gave a linear calibration plot for indinavir, and it was lower than the physiological plasma concentration index (i.e. maximum plasma concentrations, Cmax,) of indinavir (5 - 15 ng/mL) normally observed 8 h after intake. This indicates that the biosensor can be very useful in the case of ultra-rapid metabolisers where very low Cmax values are expected |
| Databáze: | OpenAIRE |
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