Epidemiology and Molecular Characterization of Streptococcus pyogenes Recovered from Scarlet Fever Patients in Central Taiwan from 1996 to 1999
| Autor: | Hsiu-Li Chang, Tzu-Hui Wang, Jui-Cheng Liao, Chien-Shun Chiou, Tsai-Ling Liao, Chun-Chin Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Microbiology (medical)
Genotype Scarlet Fever Epidemiology Streptococcus pyogenes Taiwan Biology medicine.disease_cause SmaI Microbiology Disease Outbreaks Pulsed-field gel electrophoresis medicine Humans Typing Phylogeny Molecular epidemiology biochemical phenomena metabolism and nutrition medicine.disease bacterial infections and mycoses Virology Electrophoresis Gel Pulsed-Field DNA profiling Scarlet fever bacteria |
| Popis: | One hundred seventy-nineStreptococcus pyogenesisolates recovered from scarlet fever patients from 1996 to 1999 in central Taiwan were characterized byemm, Vir, and pulsed-field gel electrophoresis (PFGE) typing methods. The protocols for Vir and PFGE typing were standardized. A database of the DNA fingerprints for the isolates was established. Nineemmoremm-like genes, 19 Vir patterns, and 26 SmaI PFGE patterns were detected among the isolates. Among the three typing methods, PFGE was the most discriminatory. However, it could not completely replace Vir typing because some isolates with identical PFGE patterns could be further differentiated into several Vir patterns. The prevalentemmtypes wereemm4 (n= 81 isolates [45%]),emm12 (n= 64 [36%]),emm1 (n= 14 [8%]), andemm22 (n= 13 [7%]). Someemmtype isolates could be further differentiated into severalemm-Vir-PFGE genotypes; however, only one genotype in eachemmgroup was usually predominant. DNA from nine isolates was resistant to SmaI digestion. Further PFGE analysis with SgrAI showed that the SmaI digestion-resistant strains could be derived from indigenous strains by horizontal transfer of exogenous genetic material. The emergence of the new strains could have resulted in an increase in scarlet fever cases in central Taiwan since 2000. Theemmsequences, Vir, and PFGE pattern database will serve as a basis for information for the long-term evolutionary study of localS. pyogenesstrains. |
| Databáze: | OpenAIRE |
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