Demonstration of a Leptin Binding Factor in Human Serum
| Autor: | Duane C. Eichler, Dorothy I. Shulman, Gregory E. Duckett, Frank B. Diamond, E. Verena Jorgensen, Allen W. Root |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Leptin
medicine.medical_specialty Biophysics Receptors Cell Surface In Vitro Techniques Biology Binding Competitive Biochemistry Iodine Radioisotopes Follicle Internal medicine medicine Animals Humans Obesity Receptor Molecular Biology Gene Leptin receptor digestive oral and skin physiology Proteins Cell Biology medicine.disease Molecular Weight Kinetics Leukemia Endocrinology Receptors Leptin Carrier Proteins hormones hormone substitutes and hormone antagonists Protein Binding Hormone |
| Zdroj: | Biochemical and Biophysical Research Communications. 233:818-822 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1997.6538 |
| Popis: | Serum leptin levels are elevated in subjects with exogenous obesity, indicating that obesity is associated with leptin resistance. Since in man no abnormalities have yet been found in either the genes for leptin or its receptor, the mechanism of leptin resistance in obesity remains unknown. To determine if resistance might be related to leptin binding by a serum component, we assessed the carrier status of leptin in serum. The presence of a specific leptin binding factor in human serum has been established by (1) demonstrating [125I]-leptin binding to a serum component that is saturable and specifically displaceable only by unlabeled leptin and not by human growth hormone, pork insulin, insulin-like growth factors I and II, luteinizing or follicle stimulating hormones, transforming growth factor-beta 1, interleukin-6, or leukemia inhibiting factor; (2) fractionating the leptin bound serum complex and the serum leptin binding component on a molecular sieving column revealing a mass of approximately 450 kDa; and (3) identifying an inverse correlation between the concentration of serum leptin and the quantity of the leptin binding component. It is suggested that binding of leptin by this serum component may influence the physiologic response to leptin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect