The RXL motif of the African cassava mosaic virus Rep protein is necessary for rereplication of yeast DNA and viral infection in plants
| Autor: | Benjamin Schäfer, Peter Rau, Katharina Hipp, Holger Jeske, Bruno Gronenborn |
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| Rok vydání: | 2014 |
| Předmět: |
DNA Replication
AC1 RXL motif viruses Clink Mutant Amino Acid Motifs Nicotiana benthamiana chemistry.chemical_compound African cassava mosaic virus Virology Plant virus Schizosaccharomyces Tobacco DNA Fungal Genetics biology Nanovirus Cell Cycle Retinoblastoma protein DNA Helicases food and beverages biology.organism_classification Yeast Rep Viral replication chemistry Begomovirus biology.protein Trans-Activators Geminivirus Mutant Proteins DNA S. pombe |
| Zdroj: | Virology. |
| ISSN: | 1096-0341 |
| Popis: | Geminiviruses, single-stranded DNA plant viruses, encode a replication-initiator protein (Rep) that is indispensable for virus replication. A potential cyclin interaction motif (RXL) in the sequence of African cassava mosaic virus Rep may be an alternative link to cell cycle controls to the known interaction with plant homologs of retinoblastoma protein (pRBR). Mutation of this motif abrogated rereplication in fission yeast induced by expression of wildtype Rep suggesting that Rep interacts via its RXL motif with one or several yeast proteins. The RXL motif is essential for viral infection of Nicotiana benthamiana plants, since mutation of this motif in infectious clones prevented any symptomatic infection. The cell-cycle link (Clink) protein of a nanovirus (faba bean necrotic yellows virus) was investigated that activates the cell cycle by binding via its LXCXE motif to pRBR. Expression of wildtype Clink and a Clink mutant deficient in pRBR-binding did not trigger rereplication in fission yeast. |
| Databáze: | OpenAIRE |
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