Origin and molecular organization of supernumerary chromosomes of Prochilodus lineatus (characiformes, prochilodontidae) obtained by DNA probes
| Autor: | Alejandro Laudicina, José Augusto Senhorini, Tatiana Aparecida Voltolin, Jehud Bortolozzi, Fábio Porto-Foresti, Claudio Oliveira, Fausto Foresti |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Population Plant Science Biology Chromosomes Evolution Molecular chemistry.chemical_compound Genetics medicine Animals education In Situ Hybridization Fluorescence B chromosome education.field_of_study medicine.diagnostic_test Hybridization probe Fishes General Medicine biology.organism_classification Chromosome microdissection Molecular biology Chromatin chemistry Insect Science Karyotyping Cytogenetic Analysis Prochilodus lineatus Animal Science and Zoology Female DNA Probes DNA Fluorescence in situ hybridization |
| Zdroj: | Genetica. 138(11-12) |
| ISSN: | 1573-6857 |
| Popis: | In Prochilodus lineatus B-chromosomes are visualized as reduced size extra elements identified as microchromosomes and are variable in morphology and number. We describe the specific total probe (B-chromosome probe) in P. lineatus obtained by chromosome microdissection and a whole genomic probe (genomic probe) from an individual without B-chromosome. The specific B-chromosome was scraped and processed to obtain DNA with amplification by DOP-PCR, and so did the genomic probe DNA. Fluorescence in situ hybridization using the B-chromosome probe labeled with dUTP-Tetramethyl-rhodamine and the genomic probe labeled with digoxigenin-FITC permitted to establish that in this species supernumerary chromosomes with varying number and morphology had different structure of chromatin when compared to that of the regular chromosomes or A complement, since only these extra elements were labeled in the metaphases. The present findings suggest that modifications in the chromatin structure of B-chromosomes to differentiate them from the A chromosomes could occur along their dispersion in the individuals of the population. |
| Databáze: | OpenAIRE |
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