A Program for Iron Economy during Deficiency Targets Specific Fe Proteins
| Autor: | Courtney E. Jahn, Graham Peers, Marinus Pilon, Michael Cantrell, Karl Ravet, Laura J. Hantzis, Gretchen E Kroh |
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| Přispěvatelé: | Colorado State University [Fort Collins] (CSU), Biochimie et Physiologie Moléculaire des Plantes (BPMP), Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), NSF : MCB 0950726, MCB 1244142, DGE-1321845, European Project: 273586,EC:FP7:PEOPLE,FP7-PEOPLE-2010-IOF,INTEGRREGULFESPLAST(2011), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
2. Zero hunger
0106 biological sciences 0301 basic medicine Physiology [SDV]Life Sciences [q-bio] Plant Science Biology biology.organism_classification Photosynthesis 01 natural sciences Electron transport chain Chloroplast 03 medical and health sciences 030104 developmental biology Sulfur assimilation Biochemistry Arabidopsis Genetics Arabidopsis thaliana [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Plastid Ferredoxin 010606 plant biology & botany |
| Zdroj: | Plant Physiology Plant Physiology, 2018, 176 (1), pp.596-610. ⟨10.1104/pp.17.01497⟩ Plant Physiology, American Society of Plant Biologists, 2018, 176 (1), pp.596-610. ⟨10.1104/pp.17.01497⟩ |
| ISSN: | 0032-0889 1532-2548 |
| Popis: | International audience; Iron (Fe) is an essential element for plants, utilized in nearly every cellular process. Because the adjustment of uptake under Fe limitation cannot satisfy all demands, plants need to acclimate their physiology and biochemistry, especially in their chloroplasts, which have a high demand for Fe. To investigate if a program exists for the utilization of Fe under deficiency, we analyzed how hydroponically grown Arabidopsis (Arabidopsis thaliana) adjusts its physiology and Fe protein composition in vegetative photosynthetic tissue during Fe deficiency. Fe deficiency first affected photosynthetic electron transport with concomitant reductions in carbon assimilation and biomass production when effects on respiration were not yet significant. Photosynthetic electron transport function and protein levels of Fe-dependent enzymes were fully recovered upon Fe resupply, indicating that the Fe depletion stress did not cause irreversible secondary damage. At the protein level, ferredoxin, the cytochrome-b(6)f complex, and Fe-containing enzymes of the plastid sulfur assimilation pathway were major targets of Fe deficiency, whereas other Fe-dependent functions were relatively less affected. In coordination, SufA and SufB, two proteins of the plastid Fe-sulfur cofactor assembly pathway, were also diminished early by Fe depletion. Iron depletion reduced mRNA levels for the majority of the affected proteins, indicating that loss of enzyme was not just due to lack of Fe cofactors. SufB and ferredoxin were early targets of transcript down-regulation. The data reveal a hierarchy for Fe utilization in photosynthetic tissue and indicate that a program is in place to acclimate to impending Fe deficiency. |
| Databáze: | OpenAIRE |
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