Amino acid content and acetylcholinesterase inhibition of Huperzia saururus infusion and decoction
| Autor: | José Luis Cabrera, Mariana Guadalupe Vallejo, María Gabriela Ortega, Jesica Ayelen Dimmer, Alicia Mariel Agnese |
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| Rok vydání: | 2013 |
| Předmět: |
Arginine
Pharmaceutical Science Decoction High-performance liquid chromatography Quantitation purl.org/becyt/ford/1 [https] chemistry.chemical_compound Drug Discovery purl.org/becyt/ford/1.4 [https] Hplc Chromatography High Pressure Liquid Nootropic Agents Pharmacology chemistry.chemical_classification Chromatography Plants Medicinal biology Dose-Response Relationship Drug Lycopodiaceae Chemistry Plant Extracts Alkaloid Otras Ciencias Químicas Ciencias Químicas Huperzia General Medicine Plant Components Aerial biology.organism_classification Chromatography Ion Exchange Acetylcholinesterase Amino acid Anti-Cholinesterase Activity Complementary and alternative medicine Molecular Medicine Spectrophotometry Ultraviolet Cholinesterase Inhibitors CIENCIAS NATURALES Y EXACTAS Phytotherapy |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 1744-5116 |
| Popis: | Huperzia saururus (Lam.) Trevis. (Lycopodiaceae), an autochthonous plant species in Argentina, is used as a memory improver in traditional medicine. It was studied for this reason and the purified alkaloid extract did show significant activity on learning and memory. The species is mostly consumed in the form of infusions and decoctions. Objectives: To evaluate the activity of the H. saururus infusion and decoction as inhibitors of acetylcholinesterase (AChE) and to determine the amino acid content in both extracts. Material and methods: Infusion and decoction were purified by ionic exchange chromatography and analyzed by high-performance liquid chromatography HPLC-UV, and the AChE inhibition of these extracts was evaluated by using the Ellman method. Results: Both infusion and decoction exerted strong AChE inhibitory activities (IC50 ¼ 7.2 0.4 and 22.7 5.6 mg/mL, respectively). Among nine amino acids, arginine (Arg) was identified in a concentration greater than 9 mg/100 g of dried aerial parts in both extracts. Discussion and conclusion: This high content of Arg could be considered a contributing factor to the activity on memory previously demonstrated for the H. saururus alkaloid extract, since Arg is implicated indirectly in mnemonic processes as a precursor in nitric oxide synthesis. Thus, the central effect of H. saururus could involve two different mechanisms, the cholinergic mechanism and the nitric oxide pathway. Fil: Vallejo, Mariana Guadalupe. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina Fil: Dimmer, Jesica Ayelen. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina Fil: Ortega, María Gabriela. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina Fil: Cabrera, Jose Luis. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina Fil: Agnese, Alicia Mariel. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina |
| Databáze: | OpenAIRE |
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