Transport media for Haemophilus ducreyi
| Autor: | Y Dangor, Ronald C. Ballard, F Radebe |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Microbiology (medical)
Sexually transmitted disease Time Factors chemistry.chemical_element Transportation Dermatology Microbiology Specimen Handling Chancroid Haemophilus ducreyi Degree Celsius Transport medium Medicine Humans Chi-Square Distribution biology business.industry Pasteurellaceae Public Health Environmental and Occupational Health Albumin Temperature biology.organism_classification female genital diseases and pregnancy complications Culture Media Infectious Diseases Hemophilus ducreyi chemistry Immunology business Selenium |
| Zdroj: | Sexually transmitted diseases. 20(1) |
| ISSN: | 0148-5717 |
| Popis: | Stuarts Amies and 4 thioglycollate/hemin-based media containing respectively selenium dioxide and albumin (SA) selenium dioxide L-glutamine and albumin (SGA) selenium dioxide and L-glutamine (SG) and L-glutamine and albumin (GA) were evaluated as transport media for Hemophilus ducreyi in a simulated laboratory-based study. No transport medium was able to maintain the viability of H. ducreyi for more than 24 hours at room temperature. Amies transport medium however maintained viability of H. ducreyi for up to 3 days and all the newly formulated transport media maintained viability of H. ducreyi for more than 4 days at 4 degrees Celsius. Subsequently swabs were taken from cases of genital ulcerations with a diagnosis of chancroid plated directly onto selective media and held at 4 degrees Celsius in the 2 most efficient transport media (SGA and GA) for various periods of time. On direct plating H. ducreyi was recovered from the genital ulcerations of 110 of 156 (71%) patients. The rates of recovery of H. ducreyi from transport media stored at 4 degrees Celsius decreased with time. Although recovery was enhanced at day 4 (117/110 >106%l) using both SGA and GA a recovery rate of 37/41 (90%) was obtained after storage in GA for 1 week. (authors) |
| Databáze: | OpenAIRE |
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