Expression of Vascular Endothelial Growth Factor-C (VEGF-C) and Its Receptor (VEGFR-3) in the Glial Reaction Elicited by Human Mesenchymal Stem Cell Engraftment in the Normal Rat Brain
| Autor: | Tae-Ryong Riew, Yoo-Jin Shin, Ha-Jin Pak, Joo-Hee Park, Mun-Yong Lee |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Pathology medicine.medical_specialty Histology Vascular Endothelial Growth Factor C Biology Mesenchymal Stem Cell Transplantation Rats Sprague-Dawley medicine Animals Humans Receptor Cells Cultured Cellular localization Neuroinflammation Microglia Macrophages Mesenchymal stem cell Brain Mesenchymal Stem Cells Articles Vascular Endothelial Growth Factor Receptor-3 medicine.disease Immunohistochemistry Rats Astrogliosis Transplantation medicine.anatomical_structure Vascular endothelial growth factor C Astrocytes Anatomy |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 63:170-180 |
| ISSN: | 1551-5044 0022-1554 |
| Popis: | To determine whether vascular endothelial growth factor-C (VEGF-C) and its receptor (VEGFR-3) are involved in the glial reaction elicited by transplanted mesenchymal stem cells (MSCs), we examined the cellular localization of VEGF-C and VEGFR-3 proteins in the striatum of adult normal rats that received bone marrow-derived human MSCs. The MSC grafts were infiltrated with activated microglia/macrophages and astrocytes over a 2-week period post-transplantation, which appeared to parallel the loss of transplanted MSCs. VEGF-C/VEGFR-3 was expressed in activated microglia/macrophages recruited to the graft site, where the induction of VEGF-C protein was rather late compared with that of its receptor. VEGF-C protein was absent or very weak on day 3, whereas VEGFR-3 immunoreactivity was evident within the first three days. Furthermore, within three days, VEGF-C could be detected in the brain macrophages localized immediately adjacent to the needle track. At the same time, almost all the brain macrophages in both regions expressed VEGFR-3. Reactive astrocytes at the graft site expressed VEGFR-3, but not VEGF-C. These data demonstrated the characteristic time- and cell-dependent expression patterns for VEGF-C and VEGFR-3 within the engrafted brain tissue, suggesting that they may contribute to neuroinflammation in MSC transplantation, possibly through the recruitment and/or activation of microglia/macrophages and astrogliosis. |
| Databáze: | OpenAIRE |
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