Studies of the formation and relase of glycerolipids by primary monolayer cultures of adult rat hepatocytes
| Autor: | Philip S. Guzelian, Craig K. Wood, Robert G. Lamb, Harold J. Fallon, Barbara Landa |
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| Rok vydání: | 1977 |
| Předmět: |
Glycerol
Male Time Factors Biophysics Phospholipid Cycloheximide Biology Biochemistry Glycerides chemistry.chemical_compound Endocrinology Glycerol Kinase medicine Animals Cells Cultured Phospholipids chemistry.chemical_classification Estradiol Ethanol Fatty acid In vitro Rats Kinetics medicine.anatomical_structure Glucose chemistry Liver Hepatocyte Glycerol-3-Phosphate O-Acyltransferase Intracellular Lipoprotein |
| Zdroj: | Biochimica et biophysica acta. 489(2) |
| ISSN: | 0006-3002 |
| Popis: | Primary monolayer culture of adult rat hepatocytes represents a relatively novel and potentially useful in vitro technique for studying various hepatic processes for extended periods of time. This study evaluates the potential of hepatocyte monolayers for studying hepatic glycerolipid biosynthesis. Hepatocyte monolayers synthesize glycerolipids from [1,3- 14 C]glycerol and fatty acids and release them into the culture medium. Glycerolipid synthesis and release by monolayers declines 40 to 50% during the first 24 h of culture and these lower rates are maintained for at least 72 h. Triacylglycerol formation and release is dependent upon fatty acid concentration in the incubation medium although phospholipid is not. The release of triacylglycerol is greater in the presence of unsaturated acids (16 : 1, 18 : 1 and 18 : 2) than saturated acids (16 : 0 and 18 : 0) although saturated acids produce equal or greater amounts of intracellular triacylglycerol. Phospholipid and triacylglycerol release is reduced in monolayers exposed to colchicine for 24 h but only triacylglycerol release is lowered by the presence of cycloheximide. These results suggest release of triacylglycerol-containing lipoprotein by hepatocyte monolayers. However, we were unable to detect known apolipoprotein in the culture medium by immunologie techniques. Exposure of hepatocyte monolayers to ethanol and estradiol causes alterations in glycerolipid production and release. These changes in glycerolipid formation correlate well with corresponding alterations in glycerol kinase activity measured in monolayer homogenates. These experiments demonstrate the potential usefulness of hepatocytes in monolayer to study various aspects of glycerolipid metabolism. Their longer viability, days rather than hours, is an advantage over other in vitro liver preparations (slices, homogenates, isolated hepatocytes and liver perfusion) for many types of experiments. |
| Databáze: | OpenAIRE |
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