Expression and Regulation of Constitutive and Acute Phase Serum Amyloid A mRNAs in Hepatic and Non‐Hepatic Cell Lines

Autor: F. C. Donoghue, R. M. O'neill, Clarissa M. Uhlar, Alexander S. Whitehead, Diana M. Steel
Rok vydání: 1996
Předmět:
Polyadenylation
animal diseases
Dexamethasone
Epithelium
KB Cells
Monocytes
HeLa
hemic and lymphatic diseases
Tumor Cells
Cultured
RNA
Neoplasm
RNA Processing
Post-Transcriptional
Cells
Cultured
Liver Neoplasms
General Medicine
Recombinant Proteins
Neoplasm Proteins
Gene Expression Regulation
Neoplastic
Endothelial stem cell
medicine.anatomical_structure
Liver
Organ Specificity
Mouth Neoplasms
Tumor necrosis factor alpha
Lymphoma
Large B-Cell
Diffuse
Half-Life
Carcinoma
Hepatocellular
Immunology
Biology
Intestinal Neoplasms
medicine
Humans
RNA
Messenger
Acute-Phase Reaction
Serum Amyloid A Protein
Messenger RNA
Interleukin-6
Tumor Necrosis Factor-alpha
Monocyte
Epithelial Cells
Fibroblasts
biology.organism_classification
Molecular biology
Adrenal Cortex Neoplasms
stomatognathic diseases
Gene Expression Regulation
Cell culture
Culture Media
Conditioned
Hepatic stellate cell
Endothelium
Vascular
HeLa Cells
Interleukin-1
Zdroj: Scandinavian Journal of Immunology. 44:493-500
ISSN: 1365-3083
0300-9475
Popis: 'Acute phase' and 'constitutive' SAA (A-SAA and C-SAA, respectively) mRNA levels were measured in hepatic and non-hepatic cell lines after treatment with monocyte conditioned medium (MoCM), with or without dexamethasone (Dex). A-SAA mRNAs were detected in MoCM-treated hepatoma cell lines (PLC/PRF/5, HuH7, HepG2, and Hep3B), a fibroblast cell line (MRC5), six epithelial cell lines (RT4/ 31, SW13, Hela Ohio, HCT-8, CaCo2, and KB), and an endothelial cell line ECV304. In KB cells, Dex alone caused a dramatic increase in A-SAA mRNA levels. C-SAA was detected in all hepatic and non-hepatic cell lines. Two differentially regulated size classes of C-SAA mRNA were detected in the hepatoma cell lines. A-SAA mRNA levels were measured in ECV304 cells treated with IL-1 beta, IL-6, TNF alpha and Dex, in various combinations, and revealed different profiles to those seen for hepatic cells. The extent of polyadenylation of A-SAA mRNA in ECV304 and KB cells differed whereas the polyadenylation of C-SAA mRNA remained constant. These data suggest that the parameters that determine the steady state mRNA levels and post-transcriptional regulation of A-SAA and C-SAA mRNAs are different and are cell type specific.
Databáze: OpenAIRE
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