A simple automated approach to measure mouse whole gut transit
| Autor: | Halil Kacmaz, David R. Linden, Kaitlyn R. Knutson, Gianrico Farrugia, Alecia Alto, Arthur Beyder, Simon J. Gibbons |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Physiology Computer science Transit time Time-Lapse Imaging Article 03 medical and health sciences Mice 0302 clinical medicine Offline analysis Animals Animal behavior Transit (astronomy) Gastrointestinal Transit Automation Laboratory Endocrine and Autonomic Systems digestive oral and skin physiology Gastroenterology Slowed transit Mice Inbred C57BL 030104 developmental biology High definition 030211 gastroenterology & hepatology Female Biomedical engineering |
| Zdroj: | Neurogastroenterol Motil |
| Popis: | Background Gastrointestinal (GI) motility is a complex physiological process that is critical for normal GI function. Disruption of GI motility frequently occurs in GI diseases or as side effects of therapeutics. Whole gut transit measurements, like carmine red leading-edge transit, in mice form the cornerstone of in vivo preclinical GI motility studies. Method We have developed an easily achievable, labor-saving method to measure whole gut transit time in mice. This approach uses inexpensive, commercially available materials to monitor pellet production over time via high definition cameras capturing time-lapse video for offline analysis. Key result We describe the assembly of our automated gut transit setup and validate this approach by comparing the results with loperamide to delay transit and conventional transit measurements. We demonstrate that compared to the control group, the loperamide group had slowed transit, evidenced by a decrease in total pellet production and prolonged whole gut transit time. The control group had an extended transit time compared with the results reported in the literature. Whole gut transit rates accelerated to times comparable to the literature by disrupting cages every 10-15 min to imitate the conventional approach, suggesting that disruption affects the assay and supports the use of an automated approach. Conclusion & inferences A novel automated, inexpensive, and easily assembled whole gut transit setup is labor-saving and allows minimal disruption to animal behavior compared with the conventional approach. |
| Databáze: | OpenAIRE |
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