A novel role for Xist RNA in the formation of a repressive nuclear compartment into which genes are recruited when silenced
| Autor: | Anton Wutz, Edith Heard, Patricia Le Baccon, Julie Chaumeil |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
RNA
Untranslated X Chromosome Transcription Genetic RNA polymerase II X-inactivation Mice Transcription (biology) Genes X-Linked X Chromosome Inactivation Genetics Animals Gene Silencing Transcription factor Repetitive Sequences Nucleic Acid Cell Nucleus biology Stem Cells RNA Cell Differentiation Non-coding RNA Embryo Mammalian RNA silencing biology.protein XIST Female RNA Long Noncoding RNA Polymerase II Developmental Biology Research Paper Transcription Factors |
| Popis: | During early mammalian female development, one of the two X chromosomes becomes inactivated. Although X-chromosome coating by Xist RNA is essential for the initiation of X inactivation, little is known about how this signal is transformed into transcriptional silencing. Here we show that exclusion of RNA Polymerase II and transcription factors from the Xist RNA-coated X chromosome represents the earliest event following Xist RNA accumulation described so far in differentiating embryonic stem (ES) cells. Paradoxically, exclusion of the transcription machinery occurs before gene silencing is complete. However, examination of the three-dimensional organization of X-linked genes reveals that, when transcribed, they are always located at the periphery of, or outside, the Xist RNA domain, in contact with the transcription machinery. Upon silencing, genes shift to a more internal location, within the Xist RNA compartment devoid of transcription factors. Surprisingly, the appearance of this compartment is not dependent on the A-repeats of the Xist transcript, which are essential for gene silencing. However, the A-repeats are required for the relocation of genes into the Xist RNA silent domain. We propose that Xist RNA has multiple functions: A-repeat-independent creation of a transcriptionally silent nuclear compartment; and A-repeat-dependent induction of gene repression, which is associated with their translocation into this silent domain. |
| Databáze: | OpenAIRE |
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