Preliminary Evidence of Endotoxin Tolerance in Dairy Cows during the Transition Period
| Autor: | Gabriele Brecchia, Massimo Amadori, Flavia Guarneri, Alessia Inglesi, Daniele Vigo, J. Filipe, Laura Menchetti, Federica Riva, Giulio Curone |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Lipopolysaccharides
Kynurenine pathway blastogenic response QH426-470 Lymphocyte Activation Peripheral blood mononuclear cell Proinflammatory cytokine Pregnancy endotoxin tolerance Placenta Genetics Animals Medicine Interleukin 6 transition period Kynurenine Genetics (clinical) biology Tumor Necrosis Factor-alpha business.industry Communication Interleukins Postpartum Period PBMC Interleukin In vitro medicine.anatomical_structure cattle Immunology Leukocytes Mononuclear biology.protein Female Tumor necrosis factor alpha business |
| Zdroj: | Genes, Vol 12, Iss 1801, p 1801 (2021) Genes |
| ISSN: | 2073-4425 |
| DOI: | 10.3390/genes12111801 |
| Popis: | The blastogenic response of bovine peripheral blood mononuclear cells (PBMCs) to lipopolysaccharides (LPS) has been investigated for a long time in our laboratories. In particular, a possible correlation between the blastogenic response to LPS and the disease resistance of dairy cows has been suggested in previous studies. Isolated PBMCs from eight cows at three different time points during the transition period (T0 = 15 days before calving; T1 = 7 days post-calving; T2 = 21 days post-calving) were cultured in the presence or absence of LPS, and the blastogenic response was assayed 72 h after in vitro stimulation. Moreover, the gene expression of proinflammatory cytokines and kynurenine pathway molecules was investigated by real-time RT-PCR on both unstimulated and stimulated PBMCs. The cows were retrospectively divided into healthy and diseased, based on the development of peripartum diseases (subclinical ketosis and placenta retention). The comparison between healthy and diseased cows suggested that healthy animals seemed to better control the response to LPS. On the contrary, diseased animals showed a much higher inflammatory response to LPS. Moreover, cows were retrospectively classified as high and low responders based on the in vitro proliferative response of PBMCs to LPS, using the median value as a threshold. Unstimulated PBMCs of low responders showed higher expression of the proinflammatory cytokines Interleukin 1-β (IL-1β), Interleukin 6 (IL-6) and Tumor Necrosis Factor-α (TNF-α), compared to high responders. Our preliminary data suggest that, during the peripartum period, high responders seem to be more tolerant to endotoxins and develop a lower inflammatory response to different stressors. Instead, low responders could be more prone to the development of unwanted inflammatory conditions in response to mild/moderate stressors. |
| Databáze: | OpenAIRE |
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