Migration versus Proliferation as Contributor to In Vitro Wound Healing of Vascular Endothelial and Smooth Muscle Cells
| Autor: | Marvin J. Slepian, Kaitlyn R. Ammann, Maxwell Li, Katrina J. DeCook |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Vascular smooth muscle Atherectomy medicine.medical_treatment Myocytes Smooth Muscle Primary Cell Culture Biology Article Extracellular matrix 03 medical and health sciences 0302 clinical medicine Cell Movement Angioplasty medicine Humans Thrombus Cell Proliferation Wound Healing Cell growth Endothelial Cells Cell Biology medicine.disease In vitro Cell biology Extracellular Matrix 030104 developmental biology 030220 oncology & carcinogenesis Wound healing Vascular Stenosis |
| Popis: | Wound closure, as a result of collective cell growth, is an essential biological response to injury. In the field of vascular biology, the response of vascular smooth muscle cells (SMCs) and endothelial cells (ECs) to injury and substrate surface is important in therapeutic clinical treatment interventions such as angioplasty and atherectomy. Specifically, the mechanism by which cells close wounds (i.e. proliferation versus migration) in response to injury stimuli is of interest to better modulate recurrent vascular stenosis, prevent thrombus formation, occlusion, and life-threatening cardiovascular events. Here, we examine growth extent and temporal sequence of events following wound or gap introduction to a confluent monolayer of vascular SMCs or ECs. Significant differences in the preferred mechanisms of these cells to close wounds or gaps were observed; after 48 h, 73% of SMC wound closure was observed to be due to proliferation, while 75% of EC wound closure resulted from migration. These mechanisms were further modulated via addition or removal of extracellular matrix substrate and injury, with ECs more responsive to substrate composition and less to injury, in comparison to SMCs. Our results indicate that ECs and SMCs heal wounds differently, and that the time and mode of injury and associated substrate surface all impact this response. |
| Databáze: | OpenAIRE |
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