Systematic Discovery of Short Linear Motifs Decodes Calcineurin Phosphatase Signaling

Autor: Anne-Claude Gingras, Cecilia Blikstad, Katharine S. Ullman, Jagoree Roy, Vikash K Yadav, Izabella Krystkowiak, Malika Amyn Kaderali, Jennifer T. Wang, Douglas R. Mackay, Ylva Ivarsson, Devin A. Bradburn, Eirini Tsekitsidou, Callie P. Wigington, Cassandra J. Wong, Tim Stearns, Su Hyun Hong, Nikhil P Damle, Martha S. Cyert, Eduard Resch, Shou-Ling Xu, Norman E. Davey
Přispěvatelé: Publica
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Mol Cell
Popis: SummaryShort linear motifs (SLiMs) drive dynamic protein-protein interactions essential for signaling, but sequence degeneracy and low binding affinities make them difficult to identify. We harnessed unbiased systematic approaches for SLiM discovery to elucidate the regulatory network of calcineurin (CN)/PP2B, the Ca2+-activated phosphatase that recognizes LxVP and PxIxIT motifs. In vitro proteome-wide detection of CN-binding peptides, in vivo SLiM-dependent proximity labeling, and in silico modeling of motif determinants uncovered unanticipated CN interactors, including NOTCH1, which we establish as a CN substrate. Unexpectedly, CN shows SLiM-dependent proximity to centrosomal and nuclear pore complex (NPC) proteins – structures where Ca2+ signaling is largely uncharacterized. CN dephosphorylates human and yeast NPC proteins and promotes accumulation of a nuclear transport reporter, suggesting conserved NPC regulation by CN. The CN network assembled here provides a resource to investigate Ca2+ and CN signaling and demonstrates synergy between experimental and computational methods, establishing a blueprint for examining SLiM-based networks.
Databáze: OpenAIRE