Enzyme activity profiles during fruit development in tomato cultivars and Solanum pennellii
| Autor: | Yves Gibon, Marie-Caroline Steinhauser, Dirk Steinhauser, Fernando Carrari, Karin Koehl, Mark Stitt, Alisdair R. Fernie |
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| Přispěvatelé: | Max Planck Institute of Molecular Plant Physiology (MPI-MP), Max-Planck-Gesellschaft, Instituto Nacional de Tecnología Agropecuaria (INTA), Station de physiologie végétale, Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
0106 biological sciences
Physiology Metabolite Biochemical Processes and Macromolecular Structures Plant Science tomato 01 natural sciences purl.org/becyt/ford/1 [https] tomate chemistry.chemical_compound plante fruitière Solanum lycopersicum Génétique des plantes Cultivar génétique quantitative 2. Zero hunger chemistry.chemical_classification 0303 health sciences education.field_of_study Principal Component Analysis biology activité enzymatique food and beverages développement du fruit profil métabolique CIENCIAS NATURALES Y EXACTAS Population Introgression Plants genetics Solanum Ciencias Biológicas [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics physiologie végétale 03 medical and health sciences fruit metabolism Biología Celular Microbiología Species Specificity Botany Genetics purl.org/becyt/ford/1.6 [https] education Sugar métabolisme 030304 developmental biology fungi biology.organism_classification Enzyme assay enzyme Enzyme solanum pennellii chemistry Fruit biology.protein 010606 plant biology & botany |
| Zdroj: | Plant Physiology Plant Physiology, American Society of Plant Biologists, 2010, 153 (1), pp.80-98. ⟨10.1104/pp.110.154336⟩ Plant Physiology 1 (153), 80-98. (2010) CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 0032-0889 1532-2548 |
| DOI: | 10.1104/pp.110.154336⟩ |
| Popis: | Enzymes interact to generate metabolic networks. The activities of more than 22 enzymes from central metabolism were profiled during the development of fruit of the modern tomato cultivar Solanum lycopersicum 'M82' and its wild relative Solanum pennellii (LA0716). In S. pennellii, the mature fruit remains green and contains lower sugar and higher organic acid levels. These genotypes are the parents of a widely used near introgression line population. Enzymes were also profiled in a second cultivar, S. lycopersicum 'Moneymaker', for which data sets for the developmental changes of metabolites and transcripts are available. Whereas most enzyme activities declined during fruit development in the modern S. lycopersicum cultivars, they remained high or even increased in S. pennellii, especially enzymes required for organic acid synthesis. The enzyme profiles were sufficiently characteristic to allow stages of development and cultivars and the wild species to be distinguished by principal component analysis and clustering. Many enzymes showed coordinated changes during fruit development of a given genotype. Comparison of the correlation matrices revealed a large overlap between the two modern cultivars and considerable overlap with S. pennellii, indicating that despite the very different development responses, some basic modules are retained. Comparison of enzyme activity, metabolite profiles, and transcript profiles in S. lycopersicum 'Moneymaker' revealed remarkably little connectivity between the developmental changes of transcripts and enzymes and even less between enzymes and metabolites. We discuss the concept that the metabolite profile is an emergent property that is generated by complex network interactions. Fil: Steinhauser, Marie Caroline. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Steinhauser, Dirk. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Koehl, Karin. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gibon, Yves. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Fernie, Alisdair R.. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Stitt, Mark. Max Planck Institute of Molecular Plant Physiology; Alemania |
| Databáze: | OpenAIRE |
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