Roles of Active-Site Aromatic Residues in Cold Adaptation of Sphingomonas glacialis Esterase EstSP1
| Autor: | Lan-Huong Tran, ChangWoo Lee, Ayesha Kashif, Sei-Heon Jang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine biology Stereochemistry Chemistry General Chemical Engineering Mutant Active site General Chemistry 01 natural sciences Esterase Article lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound Residue (chemistry) 030104 developmental biology Protein structure lcsh:QD1-999 Sphingomonas glacialis 010608 biotechnology Aromatic amino acids biology.protein Psychrophile |
| Zdroj: | ACS Omega, Vol 2, Iss 12, Pp 8760-8769 (2017) ACS Omega |
| ISSN: | 2470-1343 |
| DOI: | 10.1021/acsomega.7b01435 |
| Popis: | The aromatic amino acids, Tyr or Trp, which line the active-site walls of esterases, stabilize the catalytic His loop via hydrogen bonding. A Tyr residue is preferred in extremophilic esterases (psychrophilic or hyperthermophilic esterases), whereas a Trp residue is preferred in moderate-temperature esterases. Here, we provide evidence that Tyr and Trp play distinct roles in cold adaptation of the psychrophilic esterase EstSP1 isolated from an Arctic bacterium Sphingomonas glacialis PAMC 26605. Stern–Volmer plots showed that the mutation of Tyr191 to Ala, Phe, Trp, and His resulted in reduced conformational flexibility of the overall protein structure. Interestingly, the Y191W and Y191H mutants showed increased thermal stability at moderate temperatures. All Tyr191 mutants showed reduced catalytic activity relative to wild-type EstSP1. Our results indicate that Tyr with its phenyl hydroxyl group is favored for increased conformational flexibility and high catalytic activity of EstSP1 at low temperatures at the expense of thermal stability. The results of this study suggest that, in the permanently cold Arctic zone, enzyme activity has been selected for psychrophilic enzymes over thermal stability. The results presented herein provide novel insight into the roles of Tyr and Trp residues for temperature adaptation of enzymes that function at low, moderate, and high temperatures. |
| Databáze: | OpenAIRE |
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