Antioxidant effect of flavonoids on DCF production in HL-60 cells
| Autor: | Mahmoud A. ElSohly, Daneel Ferreira, Ahmed M. Galal, Abdel-Rahim S. Ibrahim, Samir A. Ross, Farouk S. El-Feraly, Satoshi Takamatsu |
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| Rok vydání: | 2003 |
| Předmět: |
Antioxidant
medicine.medical_treatment Leukaemia cell Microbial transformation HL-60 Cells Antioxidants law.invention Flow cytometry chemistry.chemical_compound law medicine Humans Hydrogen peroxide Respiratory Burst Flavonoids Pharmacology Plants Medicinal Dose-Response Relationship Drug medicine.diagnostic_test Chemistry Flow Cytometry Fluoresceins Respiratory burst Dose–response relationship Biochemistry Phytotherapy Oxidation-Reduction |
| Zdroj: | Phytotherapy Research. 17:963-966 |
| ISSN: | 1099-1573 0951-418X |
| Popis: | A fluorometric microplate assay was performed for the detection of respiratory burst activity in a human leukaemia cell line HL-60 by assessing oxidation of 2',7'-dichloro fluorescin diacetate (DCFH-DA). This method is based on the detection of DCFH oxidation due to the presence of hydrogen peroxide. In the present study, the antioxidant activity of a number of structurally related flavonoids of plant origin and some of their microbial transformation products (1-18) were evaluated. |
| Databáze: | OpenAIRE |
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