Preparation of nanoliposomes by microfluidic mixing in herring-bone channel and the role of membrane fluidity in liposomes formation

Autor:	Kamila Velínská, Josef Mašek, Stuart Macaulay, Milan Raska, Jaroslav Turánek, Irena Kratochvílová, Andrea Tomečková, František Hubatka, Jan Kotouček, Eliška Bartheldyová, Martina Fojtíková, Jana Stráská, D. Hrebik, Pavel Kulich, Jaroslava Bezděková
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	0301 basic medicine Membrane Fluidity Microfluidics Cholestyramine Resin Nanoparticle lcsh:Medicine Biocompatible Materials Fluorescence Polarization 02 engineering and technology Micelle Article Biomaterials 03 medical and health sciences Lab-On-A-Chip Devices Membrane fluidity Lipid bilayer lcsh:Science Liposome Multidisciplinary Nanoscale materials Chemistry Bilayer lcsh:R 021001 nanoscience & nanotechnology Nanostructures 030104 developmental biology Chemical engineering Liposomes lipids (amino acids peptides and proteins) lcsh:Q 0210 nano-technology Fluorescence anisotropy
Zdroj:	Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020) Scientific Reports
ISSN:	2045-2322
DOI:	10.1038/s41598-020-62500-2
Popis:	Introduction of microfluidic mixing technique opens a new door for preparation of the liposomes and lipid-based nanoparticles by on-chip technologies that are applicable in a laboratory and industrial scale. This study demonstrates the role of phospholipid bilayer fragment as the key intermediate in the mechanism of liposome formation by microfluidic mixing in the channel with “herring-bone” geometry used with the instrument NanoAssemblr. The fluidity of the lipid bilayer expressed as fluorescence anisotropy of the probe N,N,N-Trimethyl-4-(6-phenyl-1,3,5-hexatrien-1-yl) was found to be the basic parameter affecting the final size of formed liposomes prepared by microfluidic mixing of an ethanol solution of lipids and water phase. Both saturated and unsaturated lipids together with various content of cholesterol were used for liposome preparation and it was demonstrated, that an increase in fluidity results in a decrease of liposome size as analyzed by DLS. Gadolinium chelating lipids were used to visualize the fine structure of liposomes and bilayer fragments by CryoTEM. Experimental data and theoretical calculations are in good accordance with the theory of lipid disc micelle vesiculation.
Databáze:	OpenAIRE
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