A cocktail of affinity-purified antibodies reactive with diagnostically useful mycobacterial antigens ES-31, ES-43, and EST-6 for detecting the presence of Mycobacterium tuberculosis
| Autor: | Santa Saha Roy, Vijay Upadhye, Bhaskar C Harinath, Niraj Shende, Suvarna Hirudkar, Satish Kumar |
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| Rok vydání: | 2006 |
| Předmět: |
Microbiology (medical)
Affinity purified antibody Tuberculosis animal diseases Enzyme-Linked Immunosorbent Assay Antigen-Antibody Complex Biology Chromatography Affinity Mycobacterium tuberculosis Antigen Antibody Specificity medicine Humans Tuberculosis Pulmonary Antigens Bacterial Sputum General Medicine medicine.disease biology.organism_classification Antibodies Bacterial Virology Immune complex Infectious Diseases Mycobacterial antigen biology.protein Electrophoresis Polyacrylamide Gel Antibody Bacteria Chromatography Liquid |
| Zdroj: | Diagnostic Microbiology and Infectious Disease. 55:65-68 |
| ISSN: | 0732-8893 |
| DOI: | 10.1016/j.diagmicrobio.2005.10.021 |
| Popis: | A cocktail of affinity-purified antibodies against diagnostically useful Mycobacterium tuberculosis H37Ra excretory-secretory protein antigens ES-31, ES-43, and EST-6 was explored for detection of circulating free and immune-complexed (IC) antigen in sera of patients with confirmed tuberculosis (TB) by sandwich enzyme-linked immunosorbent assay and compared with monospecific anti-ES-31 antibody. Out of 68 smear-positive TB cases studied, using cocktail antibody, a sensitivity of 97% (66/68) for immune-complexed cocktail antigen and 91% (62/68) for free cocktail-antigen detection was observed, compared to 91% (62/68) for immune-complexed ES-31 and 79% (54/68) for free ES-31 antigen when anti-ES-31 antibody was used alone. Thus, combinatorial use of antibodies showed improved sensitivity and was thus observed to be better than single antibody. The specificity was observed to be 99% for immune-complexed antigen using cocktail antibody. Furthermore, analysis of different groups of TB sera showed that circulating immune-complexed antigen is a sensitive marker than free antigen. |
| Databáze: | OpenAIRE |
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