Sulfation of extracellular matrices modifies responses of alveolar type II cells to fibroblast growth factors
| Autor: | Philip L. Sannes, Jody Khosla, Pi Wan Cheng |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Pulmonary and Respiratory Medicine
Physiology medicine.medical_treatment Basic fibroblast growth factor Biology Fibroblast growth factor Basement Membrane chemistry.chemical_compound Sulfation Physiology (medical) medicine Animals Chondroitin sulfate Hyaluronic Acid Growth Substances Fibroblast Cells Cultured Epidermal Growth Factor Heparin Growth factor Chondroitin Sulfates DNA Cell Biology Rats Inbred F344 Extracellular Matrix Rats Cell biology Pulmonary Alveoli Kinetics Chemically defined medium medicine.anatomical_structure Bromodeoxyuridine chemistry Biochemistry Fibroblast Growth Factor 1 Fibroblast Growth Factor 2 Pulmonary alveolus |
| Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology. 271:L688-L697 |
| ISSN: | 1522-1504 1040-0605 |
| DOI: | 10.1152/ajplung.1996.271.5.l688 |
| Popis: | The pulmonary alveolar basement membrane (BM) associated with alveolar type II cells has been shown to be significantly less sulfated than that of type I cells. To examine the biological significance of this observation, we measured the incorporation of 5-bromodeoxyuridine (BrdU) as an indicator of DNA synthesis in isolated rat type II cells cultured for 72-120 h on substrata that were naturally sulfated, not sulfated, or chemically desulfated in serum-free, hormonally defined media, with and without selected growth factors. The percentage of cells incorporating BrdU was significantly elevated by desulfated chondroitin sulfate in the presence of fibroblast growth factor-2 (FGF-2 or basic FGF) and depressed by heparin in the presence of either FGF-1 or acidic FGF or FGF-2. This depressive effect was lost by removing sulfate from the heparin. Some responses were dependent on the period of time in culture and concentration and molecular weight of the substrata. These observations support the notion that sulfation per se of certain components of BM is a key determinant of type II cell responses to select growth factors that may define patterns of proliferation and differentiation. |
| Databáze: | OpenAIRE |
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