A single-cell atlas of Plasmodium falciparum transmission through the mosquito
| Autor: | Virginia M. Howick, Jake Baum, Adam J. Reid, Clare Andradi-Brown, Juliana Cudini, Mara K. N. Lawniczak, Eliana Real, Mira S. Davidson, Kathrin Witmer, Farah A. Dahalan, Sunil Kumar Dogga, Joshua Blight |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Science Plasmodium falciparum Cell General Physics and Astronomy Virulence Host tropism Computational biology General Biochemistry Genetics and Molecular Biology Article Transcriptome 03 medical and health sciences 0302 clinical medicine Malaria transmission parasitic diseases medicine Animals Parasites Gene 030304 developmental biology 0303 health sciences Multidisciplinary biology Transmission (medicine) 030306 microbiology RNA sequencing General Chemistry medicine.disease biology.organism_classification Malaria 3. Good health Parasite biology 030104 developmental biology medicine.anatomical_structure 030217 neurology & neurosurgery |
| Zdroj: | Nature Communications Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021) |
| ISSN: | 2041-1723 |
| Popis: | Malaria parasites have a complex life cycle featuring diverse developmental strategies, each uniquely adapted to navigate specific host environments. Here we use single-cell transcriptomics to illuminate gene usage across the transmission cycle of the most virulent agent of human malaria - Plasmodium falciparum. We reveal developmental trajectories associated with the colonization of the mosquito midgut and salivary glands and elucidate the transcriptional signatures of each transmissible stage. Additionally, we identify both conserved and non-conserved gene usage between human and rodent parasites, which point to both essential mechanisms in malaria transmission and species-specific adaptations potentially linked to host tropism. Together, the data presented here, which are made freely available via an interactive website, provide a fine-grained atlas that enables intensive investigation of the P. falciparum transcriptional journey. As well as providing insights into gene function across the transmission cycle, the atlas opens the door for identification of drug and vaccine targets to stop malaria transmission and thereby prevent disease. Here the authors use single-cell RNA-seq to profile the transmission stages of the human malaria parasite Plasmodium falciparum as it progresses through the Anopheles mosquito. They highlight unique patterns of gene usage throughout this development and identify potential pleiotropic genes that function at multiple life cycle stages. |
| Databáze: | OpenAIRE |
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