Genotoxic Effect in Autoimmune Diseases Evaluated by the Micronucleus Test Assay: Our Experience and Literature Review
| Autor: | Aurelio Flores-García, Nicole Macriz Romero, María Luisa Ramos Ibarra, Penélope Valdez Aburto, Olivia Torres-Bugarín, María Guadalupe Zavala-Cerna |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
DNA damage
Mitosis lcsh:Medicine Apoptosis DNA Fragmentation Review Article Biology Systemic inflammation medicine.disease_cause General Biochemistry Genetics and Molecular Biology Autoimmune Diseases Neoplasms medicine Lymphocytes Cell Proliferation Cell Nucleus Micronucleus Tests General Immunology and Microbiology lcsh:R Autoantibody General Medicine Oxidative Stress Immunology Micronucleus test Carcinogens DNA fragmentation Biomarker (medicine) medicine.symptom Micronucleus Genotoxicity DNA Damage Mutagens |
| Zdroj: | BioMed Research International, Vol 2015 (2015) BioMed Research International |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | Autoimmune diseases (AD) are classified into organ-specific, systemic, and mixed; all forms of AD share a high risk for cancer development. In AD a destructive immune response induced by autoreactive lymphocytes is started and continues with the production of autoantibodies against different targets; furthermore apoptosis failure and loss of balance in oxidative stress as a consequence of local or systemic inflammation are common features seen in AD as well. Micronucleus (MN) assay can be performed in order to evaluate loss of genetic material in a clear, accurate, fast, simple, and minimally invasive test. The MN formation in the cytoplasm of cells that have undergone proliferation is a consequence of DNA fragmentation during mitosis and the appearance of small additional nuclei during interphase. The MN test, widely accepted forin vitroandin vivogenotoxicity research, provides a sensitive marker of genomic damage associated to diverse conditions. In here, we present a review of our work and other published papers concerning genotoxic effect in AD, identified by means of the MN assay, with the aim of proposing this tool as a possible early biomarker for genotoxic damage, which is a consequence of disease progression. Additionally this biomarker could be used for follow-up, to asses genome damage associated to therapies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|