Early proliferation of CCR5+ CD38+++ antigen-specific CD4+ Th1 effector cells during primary HIV-1 infection
| Autor: | Mee Ling Munier, Daniel Kaufmann, John Zaunders, Eric S. Rosenberg, Dick Quan, Tim Ramacciotti, John M. Kaldor, Pat Grey, Bruce D. Walker, David A. Cooper, Susanna Ip, Robert Finlayson, Don Smith, Anthony D. Kelleher |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Receptors CCR5 Immunology HIV Infections CD38 Biology Biochemistry Interleukin 21 Antigen Antigens CD Aldesleukin Interferon medicine Humans Cytotoxic T cell ADP-ribosyl Cyclase Cell Proliferation Membrane Glycoproteins Cell Biology Hematology T lymphocyte Th1 Cells ADP-ribosyl Cyclase 1 Virology Granzyme B Phenotype HIV-1 medicine.drug |
| Zdroj: | Blood. 106:1660-1667 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood-2005-01-0206 |
| Popis: | We investigated whether HIV-1 antigen-specific CD4+ T cells expressed the viral coreceptor CCR5 during primary HIV-1 infection (PHI). In the peripheral blood of subjects with very early PHI (< 22 days after onset of symptoms), there was a 10- to 20-fold increase in the proportion of highly activated (CD38+++) and proliferating (Ki-67+) CD4+ T cells that expressed CCR5+, and were mostly T-cell intracellular antigen-1 (TIA-1)+ perforin+ granzyme B+. Inthe same patient samples, CD4+ T cells producing interferon (IFN)–γ in response to HIV group-specific antigen (Gag) peptides were readily detected (median, 0.58%) by intracellular cytokine assay—these cells were again predominantly CD38+++, Ki-67+, and TIA-++, as well as Bcl-2low. On average, 20% of the Gag-specific CD4+ T cells also expressed interleukin-2 (IL-2) and were CD127 (IL-7R)+. Taken together, these results suggest that Gag-specific T-helper 1 (Th1) effector cells express CCR5 during the primary response and may include precursors of long-term self-renewing memory cells. However, in PHI subjects with later presentation, antigen-specific CD4+ T cells could not be readily detected (median, 0.08%), coinciding with a 5-fold lower level of the CCR5+CD38+++ CD4+ T cells. These results suggest that the antiviral response to HIV-1 infection includes highly activated CCR5+CD4+ cytotoxic effector cells, which are susceptible to both apoptosis and cytopathic infection with HIV-1, and rapidly decline. |
| Databáze: | OpenAIRE |
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