Inhibition of nuclear entry of HPV16 pseudovirus-packaged DNA by an anti-HPV16 L2 neutralizing antibody
| Autor: | Takamasa Takeuchi, Tadahito Kanda, Keiko Tanaka, Kazunari Kondo, Seiichiro Mori, Yoshiyuki Ishii |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
HPV16
Cell Active Transport Cell Nucleus Anti-P56/75 Antibodies Viral Cell Line HeLa Viral entry Virology medicine Animals Humans Neutralizing antibody Cell Nucleus Human papillomavirus 16 biology Virus Assembly Papillomavirus Infections Oncogene Proteins Viral L2 biology.organism_classification Antibodies Neutralizing Molecular biology Nuclear DNA Cytosol medicine.anatomical_structure Cell culture DNA Viral biology.protein Capsid Proteins Rabbits Antibody HeLa Cells |
| Zdroj: | Virology. 406:181-188 |
| ISSN: | 0042-6822 |
| Popis: | Rabbit anti-HPV16 L2 serum (anti-P56/75) neutralizes multiple oncogenic human papillomaviruses (HPVs). We inoculated HeLa cells with HPV16 pseudovirus (16PV) and with anti-P56/75-bound 16PV (16PV-Ab). Both 16PV and 16PV-Ab attached equally well to the cell surface. However, the cell-attached L1 protein of 16PV became trypsin-resistant after incubation at 37°C, whereas approximately 20% of the cell-attached 16PV-Ab L1 remained trypsin-sensitive. Confocal microscopy of HeLa cells inoculated with 16PV revealed packaged DNA in the nucleus at 22h after inoculation; however, nuclear DNA was not detected in cells inoculated with 16PV-Ab. Electron microscopy of HeLa cells inoculated with 16PV showed particles located in multivesicular bodies, lamellar bodies, and the cytosol after 4h; no cytosolic particles were detected after inoculation with 16PV-Ab. These data suggest that anti-P56/75 inhibits HPV infection partly by blocking viral entry and primarily by blocking the transport of the viral genome to the nucleus. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect