Facile synthesis of Au/ZnO/Ag nanoparticles using Glechoma hederacea L. extract, and their activity against leukemia
| Autor: | Aleksandra Romaniuk-Drapała, Renata Dobrucka, Mariusz Kaczmarek |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Silver
Scanning electron microscope Biomedical Engineering Nanoparticle Metal Nanoparticles 02 engineering and technology Jurkat cells Article Metal Au/ZnO/Ag nanoparticles 03 medical and health sciences Synthesis Spectroscopy Fourier Transform Infrared Humans Viability assay Fourier transform infrared spectroscopy Molecular Biology Glechoma hederacea L 030304 developmental biology Cancer 0303 health sciences Leukemia Lamiaceae Chemistry Plant Extracts 021001 nanoscience & nanotechnology Anti-Bacterial Agents Transmission electron microscopy Cell culture visual_art visual_art.visual_art_medium Leukocytes Mononuclear Zinc Oxide 0210 nano-technology Nuclear chemistry |
| Zdroj: | Biomedical Microdevices |
| ISSN: | 1572-8781 1387-2176 |
| Popis: | Metal combinations have been attracting the attention of scientists for some time. They usually exhibit new characteristics that are different from the ones possessed by their components. In this work, Au/ZnO/Ag nanoparticles were synthesized biologically using Glechoma hederacea L. extract. The synthesized Au/ZnO/Ag nanoparticles were characterized by UV-Vis, Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM), and Atomic Force Microscopy (AFM). The microscopic methods confirmed the presence of spherical nanoparticles of 50–70 nm. The influence of biologically synthesized Au/ZnO/Ag nanoparticles on the vitality of human cells was evaluated in vitro with the use of established human Acute T Cell Leukemia cell line, Jurkat (ATCC® TIB-152™), as well as mononuclear cells isolated from peripheral blood (PBMC) of voluntary donors. Cell survival and the half-maximal inhibitory concentration index (IC50) were analyzed by the MTT test. The studies showed that the total loss of cell viability occurred at the Au/ZnO/Ag nanoparticle concentration range of 10 µmol–50 µmol. The use of Au/ZnO/Ag nanoparticles at the concentration of 100 µmol eliminated almost all living cells from the culture in 24h. The above observation confirms the result obtained during the MTT test. |
| Databáze: | OpenAIRE |
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