Counting cycles of EF-Tu to measure proofreading in translation
| Autor: | Charles G. Kurland, Måns Ehrenberg, Tarmo Ruusala |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Discrete mathematics
Models Genetic Chemistry Phenylalanine General Medicine Peptide Elongation Factor Tu RNA Transfer Amino Acyl Translation (geometry) Guanosine Diphosphate Biochemistry Measure (mathematics) Kinetics Crystallography Leucine Protein Biosynthesis In vitro system Thermodynamics Proofreading Kinetic proofreading Mathematics EF-Tu |
| Zdroj: | Biochimie. 68:261-273 |
| ISSN: | 0300-9084 |
| DOI: | 10.1016/s0300-9084(86)80023-2 |
| Popis: | Summary A new method (T. Ruusala et al., 1982, EMBO J. 1, 75–78, 741–748) for analyzing kinetic proofreading in translation is described. An in vitro system is arranged so that its rate of polypeptide synthesis is determined by the release rate of GDP from EF-Tu in the absence of EF-Ts. This enables the counting of the number of EF-Tu cycles for correct as well as for incorrect peptide bonds. The necessary equations are derived and the approximations involved in these are discussed together with data from experiments not previously described. |
| Databáze: | OpenAIRE |
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