Purification of human alpha-2-macroglobulin by chromatography on Cibacron Blue Sepharose
| Autor: | P.K. Hall, R.C. Roberts, James Travis, G.D. Virca |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Chromatography
Chemistry Sepharose Size-exclusion chromatography Biophysics Albumin Cell Biology Biochemistry Blood proteins Chromatography Affinity Affinity chromatography Human plasma Homogeneous Chromatography Gel Methods alpha-Macroglobulins Cibacron blue-sepharose Coloring Agents Molecular Biology α 2 macroglobulin |
| Zdroj: | Analytical biochemistry. 89(1) |
| ISSN: | 0003-2697 |
| Popis: | A simple and efficient procedure has been devised for the isolation of α-2-macroglobulin from human plasma (type 1-1 haptoglobulin). The primary step is gel filtration and affinity chromatography on Cibacron Blue Sepharose, which selectively removes albumin and retards lipoproteins and γ-globulin, while effecting the molecular sieving of the remainder of the plasma proteins. This results in the separation of about 40% of the α-2-macroglobulin as a homogeneous component. A second step, gel filtration on Ultrogel AcA 22, may be utilized to separate α-2-macroglobulin in contaminated fractions obtained after Cibacron Blue Sepharose chromatography. |
| Databáze: | OpenAIRE |
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