Isolation and genomic characterization of a novel orthoreovirus from a brown-eared bulbul (Hypsipetes amaurotis) in Japan
| Autor: | Rikio Kirisawa, Yoshitaka Ogasawara, Hiromi Ueda, Naoya Kikuchi |
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| Rok vydání: | 2015 |
| Předmět: |
Sequence analysis
Orthoreovirus Avian viruses Molecular Sequence Data Sequence Homology Cell Line Japan Phylogenetics Virology Animals Cluster Analysis Humans Passeriformes Orthoreovirus Phylogeny biology Phylogenetic tree Virion virus diseases Sequence Analysis DNA Bulbul biology.organism_classification Avian orthoreovirus Microscopy Electron Hypsipetes amaurotis Novel virus RNA Viral |
| Zdroj: | Journal of General Virology. 96:1777-1786 |
| ISSN: | 1465-2099 0022-1317 |
| Popis: | Five species, Mammalian orthoreovirus, Avian orthoreovirus (ARV), Nelson Bay orthoreovirus (NBV), Baboon orthoreovirus and Reptilian orthoreovirus, have been identified in the genus Orthoreovirus. Their genomes each consist of 10 dsRNA segments. A novel orthoreovirus was isolated from the haemorrhagic intestine of a dead brown-eared bulbul (Hypsipetes amaurotis) in Japan. The virus formed syncytia in Caco-2 and Vero cells. Electron microscopy revealed non-enveloped capsids of ~70 nm diameter, which were characteristic of reoviruses. Complete genomic sequences were determined. The S1 segment was tricistronic and encoded three proteins, p10, p17 and σC, as in the two species ARV and NBV. Sequence and phylogenetic analyses showed that the virus was similar to ARV and NBV, but was located on a phylogenetic branch different from that of ARV and NBV. The virus had the closest phylogenetic relationship to two reovirus strains: SSRV from a Steller sea lion in Canada and PsRV Ge01 from a psittaciform bird in Europe. The 10 RNA segments had a 3' pentanucleotide sequence (UCAUC-3') conserved amongst all members of the genus Orthoreovirus, and a unique 5' terminal heptasequence (5'-GCUUUUC) that was the same as those of SSRV and PsRV Ge01. These results suggested that the novel virus might form a new species with the two strains in the genus Orthoreovirus. |
| Databáze: | OpenAIRE |
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