Water Extract of Lotus Leaf Alleviates Dexamethasone-Induced Muscle Atrophy via Regulating Protein Metabolism-Related Pathways in Mice

Autor: Dong-Seon Kim, Jung-Eun Kim, Jae Youl Cho, Han Gyung Kim, Su-Young Choi, Jeong-Ho Geum, Minkyeong Jo, Jieun Oh, Yoonyong Yang, Sang Hee Park, Keejung Yoon, Jin Kyeong Kim, Ji Hye Kim
Rok vydání: 2020
Předmět:
Quercetin 3-O-beta-glucuronide
Male
Nelumbo nucifera Gaertn
Protein metabolism
Pharmaceutical Science
leaf of lotus
Dexamethasone
Analytical Chemistry
Mice
chemistry.chemical_compound
0302 clinical medicine
Drug Discovery
Chromatography
High Pressure Liquid
0303 health sciences
Kinase
MuRF1
dexamethasone-induced muscle atrophy
Muscle atrophy
Muscular Atrophy
Chemistry (miscellaneous)
030220 oncology & carcinogenesis
C2C12 myoblast cells
Molecular Medicine
Phosphorylation
medicine.symptom
medicine.medical_specialty
Blotting
Western
Real-Time Polymerase Chain Reaction
Article
Cell Line
lcsh:QD241-441
03 medical and health sciences
lcsh:Organic chemistry
Internal medicine
medicine
Animals
Physical and Theoretical Chemistry
Muscle
Skeletal
Protein kinase B
Atrogin-1
PI3K/AKT/mTOR pathway
030304 developmental biology
Plant Extracts
Organic Chemistry
Water
AMPK
muscle wasting
X-Ray Microtomography
medicine.disease
Plant Leaves
Endocrinology
chemistry
Sarcopenia
Lotus
Zdroj: Molecules, Vol 25, Iss 4592, p 4592 (2020)
Molecules
Volume 25
Issue 20
ISSN: 1420-3049
DOI: 10.3390/molecules25204592
Popis: Muscle atrophy is an abnormal condition characterized by loss of skeletal muscle mass and function and is primarily caused by injury, malnutrition, various diseases, and aging. Leaf of lotus (Nelumbo nucifera Gaertn), which has been used for medicinal purposes, contains various active ingredients, including polyphenols, and is reported to exert an antioxidant effect. In this study, we investigated the effect of water extract of lotus leaf (LL) on muscle atrophy and the underlying molecular mechanisms of action. Amounts of 100, 200, or 300 mg/kg/day LL were administered to dexamethasone (DEX)-induced muscle atrophy mice for 4 weeks. Micro-computed tomography (CT) analysis revealed that the intake of LL significantly increased calf muscle volume, surface area, and density in DEX-induced muscle atrophy mice. Administration of LL recovered moving distance, grip strength, ATP production, and body weight, which were decreased by DEX. In addition, muscle damage caused by DEX was also improved by LL. LL reduced the protein catabolic pathway by suppressing gene expression of muscle atrophy F-Box (MAFbx
atrogin-1), muscle RING finger 1 (MuRF1), and forkhead box O (FoxO)3a, as well as phosphorylation of AMP-activated kinase (AMPK). The AKT-mammalian target of the rapamycin (mTOR) signal pathway, which is important for muscle protein synthesis, was increased in LL-administered groups. The HPLC analysis and pharmacological test revealed that quercetin 3-O-beta-glucuronide (Q3G) is a major active component in LL. Thus, Q3G decreased the gene expression of atrogin-1 and MuRF1 and phosphorylation of AMPK. This compound also increased phosphorylation levels of mTOR and its upstream enzyme AKT in DEX-treated C2C12 cells. We identified that LL improves muscle wasting through regulation of muscle protein metabolism in DEX-induced muscle atrophy mice. Q3G is predicted to be one of the major active phenolic components in LL. Therefore, we propose LL as a supplement or therapeutic agent to prevent or treat muscle wasting, such as sarcopenia.
Databáze: OpenAIRE
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