Dose‐dependant acute or subacute disease caused byBurkholderia pseudomalleistrain NCTC 13392 in a BALB/c aerosol model of infection
| Autor: | Julia A. Tree, Simon R. Bate, Julia Vipond, Graham Hall, Allen D. G. Roberts, Geoffrey Pearson, Emma Rayner, Simon G. P. Funnell, Graham J. Hatch |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Burkholderia pseudomallei
Melioidosis aerosol Burkholderia Virulence Spleen Disease Applied Microbiology and Biotechnology BALB/c Microbiology Mice 03 medical and health sciences medicine Animals subacute Aerosolization 030304 developmental biology Aerosols Mice Inbred BALB C 0303 health sciences biology 030306 microbiology business.industry pseudomallei dose Original Articles General Medicine biology.organism_classification medicine.disease Public Health Microbiology/Clinical Microbiology Disease Models Animal medicine.anatomical_structure Original Article business Biotechnology |
| Zdroj: | Journal of Applied Microbiology |
| ISSN: | 1365-2672 1364-5072 |
| DOI: | 10.1111/jam.14396 |
| Popis: | Aims The goal of this study was to examine, for the first time, the virulence and pathogenicity of aerosolized Burkholderia pseudomallei, strain NCTC 13392, in BALB/c mice in order to develop an animal model for testing novel medical countermeasures (MCMs) for the treatment of human acute and subacute (a disease state between acute and chronic) melioidosis. Methods and Results BALB/c mice were exposed to varying doses of aerosolized bacteria. Acute disease was seen in animals exposed to a very‐high dose (≥103 CFU per animal) and death occurred 3–4 days postchallenge (pc). Bacteria were detected in the lungs, liver, kidney and spleen. In contrast, animals exposed to a low dose ( |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text