Tissue Specificity of Cross-Reactive Allogeneic Responses by EBV EBNA3A-Specific Memory T Cells
| Autor: | Marry E.I. Franke-van Dijk, Jamie Rossjohn, Dave L. Roelen, Ilias I.N. Doxiadis, Lloyd D'Orsogna, Ellen M.W. van der Meer-Prins, Arend Mulder, Pieter van der Pol, M. Eikmans, J. Anholts, Frans H. J. Claas, James McCluskey, Cees van Kooten |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Herpesvirus 4
Human Clone (cell biology) Human leukocyte antigen Biology CD8-Positive T-Lymphocytes Cross Reactions medicine.disease_cause Umbilical vein Memory T cells HLA-B44 Antigen Kidney Tubules Proximal Heterologous immunity Antigen EBV medicine Cytotoxic T cell Humans Alloreactivity Cells Cultured Transplantation T lymphocyte Epstein–Barr virus Molecular biology Tissue specificity Epstein-Barr Virus Nuclear Antigens HLA-B Antigens Organ Specificity ATP-Binding Cassette Transporters Endothelium Vascular Peptides |
| Zdroj: | Transplantation, 91(5), 494-500 |
| DOI: | 10.1097/tp.0b013e318207944c |
| Popis: | Background: The crossreactivity of Epstein-Barr virus (EBV Epstein-Barr virus nuclear antigen 3A [EBNA3A])-specific CD8 T cells against allogeneic human leukocyte antigen (HLA)-B*44:02 has been shown to be dependent on presentation of self-peptide EEYLQAFTY by the target antigen. In this study, we report that allogeneic HLA-B*44:02+ proximal tubular epithelial cells (PTECs) and human umbilical vein endothelial cells (HUVECs) are poor targets for EBV EBNA3A-specific T cells. Methods: The EEY peptide was exogenously loaded onto HLA-B*44:02 and HLA-B*44:03-expressing PTECs and HUVECs. EEY-peptide-loaded, and unloaded, PTECs and HUVECs were then incubated with serial dilutions of our EBNA3A T-cell clone, in a cytotoxicity assay. Results: Although HLA-B*44:02-expressing PTECs were specifically lysed in proportion to the effector/target ratio by the EBNA3A T-cell clone, without peptide loading, lysis was greatly increased by exogenous EEY peptide loading (15% vs. 75%; P |
| Databáze: | OpenAIRE |
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