Pseudomonas aeruginosa killing of Caenorhabditis elegans used to identify P. aeruginosa virulence factors
| Autor: | Laurence G. Rahme, Frederick M. Ausubel, Jeffrey A. Sternberg, Man-Wah Tan, Ronald G. Tompkins |
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| Rok vydání: | 1999 |
| Předmět: |
Molecular Sequence Data
Mutant Arabidopsis Virulence Biology medicine.disease_cause Microbiology Mice Bacterial Proteins Genes Regulator Escherichia coli medicine Animals Humans Pseudomonas Infections Caenorhabditis elegans Gene DNA Primers Mammals Genetics Azotobacter vinelandii Multidisciplinary Base Sequence Pseudomonas aeruginosa Genetic Complementation Test Biological Sciences biology.organism_classification Neisseria gonorrhoeae DNA-Binding Proteins Mutagenesis Insertional DNA Transposable Elements Trans-Activators bacteria Transcription Factors |
| Zdroj: | Proceedings of the National Academy of Sciences. 96:2408-2413 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.96.5.2408 |
| Popis: | We reported recently that the human opportunistic pathogen Pseudomonas aeruginosa strain PA14 kills Caenorhabditis elegans and that many P. aeruginosa virulence factors (genes) required for maximum virulence in mouse pathogenicity are also required for maximum killing of C. elegans. Here we report that among eight P. aeruginosa PA14 Tn phoA mutants isolated that exhibited reduced killing of C. elegans , at least five also exhibited reduced virulence in mice. Three of the Tn phoA mutants corresponded to the known virulence-related genes lasR, gacA , and lemA . Three of the mutants corresponded to known genes ( aefA from Escherichia coli, pstP from Azotobacter vinelandii , and mtrR from Neisseria gonorrhoeae ) that had not been shown previously to play a role in pathogenesis, and two of the mutants contained Tn phoA inserted into novel sequences. These data indicate that the killing of C. elegans by P. aeruginosa can be exploited to identify novel P. aeruginosa virulence factors important for mammalian pathogenesis. |
| Databáze: | OpenAIRE |
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