Simplified method for measuring urinary leukotriene E4
| Autor: | Diwen Qiu, T.K. Lim, Chee-Wee Lee, Wan C. Tan, K.P. Hui |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Adult
Leukotriene E4 Respiratory Distress Syndrome Chromatography medicine.diagnostic_test Urinary system Radioimmunoassay General Chemistry Urine respiratory system High-performance liquid chromatography Asthma Immunoenzyme Techniques chemistry.chemical_compound chemistry Leukotriene E Immunoassay medicine Humans Quantitative analysis (chemistry) Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 677:152-155 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/0378-4347(95)00468-8 |
| Popis: | The conventional method for measuring urinary leukotriene E 4 (LTE4) is by reversed-phase high-performance liquid chromatography (RP-HPLC), followed by radioimmunoassay (RIA) or enzyme immunoassay (EIA). We measured urinary LTE4 levels by two methods, HPLC with EIA and EIA alone after initial crude extraction of urine using an octadecyl reversed-phase extraction cartridge (Sep-Pak). Ninety-three urine samples from normal subjects and patients with bronchial asthma and adult respiratory distress syndrome were tested. The results showed that urinary LTE4 levels measured by EIA significantly correlated with those measured by HPLC plus EIA in the three groups ( r =0.88, 0.85, 0.68). The absolute values of urinary LTE4 measured by EIA without HPLC purification were higher than by EIA with HPLC purification. This suggests that HPLC may not be necessary for routine urinary LTE4 quantitation in different clinical situations. |
| Databáze: | OpenAIRE |
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