Endoplasmic reticulum stress triggers ROS signalling, changes the redox state, and regulates the antioxidant defence of Arabidopsis thaliana

Autor: Ismail Turkan, Askim Hediye Sekmen, Baris Uzilday, Rengin Ozgur
Přispěvatelé: Ege Üniversitesi
Rok vydání: 2014
Předmět:
0106 biological sciences
Protein Folding
Physiology
Arabidopsis
Plant Science
Oxidative phosphorylation
Sodium Chloride
Protein oxidation
medicine.disease_cause
Plant Roots
01 natural sciences
Antioxidants
03 medical and health sciences
chemistry.chemical_compound
tunicamycin
medicine
oxidative stress
Arabidopsis thaliana
Plant Proteins
salt stress
030304 developmental biology
0303 health sciences
biology
Arabidopsis Proteins
Reverse Transcriptase Polymerase Chain Reaction
Endoplasmic reticulum
Antioxidant defence
Hydrogen Peroxide
ROS signalling
Tunicamycin
biology.organism_classification
3. Good health
Cell biology
chemistry
Plant Stomata
endoplasmic reticulum stress
Unfolded protein response
Signal transduction
Reactive Oxygen Species
Oxidation-Reduction
Oxidative stress
Research Paper
Signal Transduction
010606 plant biology & botany
Zdroj: Journal of Experimental Botany
ISSN: 1460-2431
0022-0957
DOI: 10.1093/jxb/eru034
Popis: WOS: 000334099900012
PubMed ID: 24558072
Endoplasmic reticulum stress, which is induced by tunicamycin, triggers reactive oxygen species signalling via NADPH oxidase activity and also regulates the antioxidant defence system in Arabidopsis thaliana.Inefficient chaperone activity in endoplasmic reticulum (ER) causes accumulation of unfolded proteins and is called ER stress, which triggers the unfolded protein response. For proper oxidative protein folding, reactive oxygen species (ROS) such as H2O2 are produced in the ER. Although the role of ROS during abiotic stresses such as salinity is well documented, the role of ER-related ROS production and its signalling is not yet known. Moreover, how H2O2 production, redox regulation, and antioxidant defence are affected in salt-treated plants when ER protein-folding machinery is impaired needs to be elucidated. For this aim, changes in NADPH-oxidase-dependent ROS signalling and H2O2 content at sequential time intervals and after 48h of ER stress, induced by tunicamycin (Tm), salinity, and their combination were determined in Arabidopsis thaliana. The main root growth was inhibited by ER stress, while low levels of Tm caused an increase in lateral root density. Salt stress and Tm induced the expression of ER-stress-related genes (bZIP17, bZIP28, bZIP60, TIN1, BiP1, BiP3) and ERO1. Tm induced expression of RBOHD and RBOHF, which led to an early increase in H2O2 and triggered ROS signalling. This study is the first report that ER stress induces the antioxidant system and the AsadaHalliwell pathway of A. thaliana in a similar way to salinity. ER stress caused oxidative damage, as evident by increased H2O2 accumulation, lipid peroxidation, and protein oxidation. As a result, this study shows that ER stress triggers ROS signalling, changes the redox state, and regulates the antioxidant defence of A. thaliana.
Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [212T018]; Ege University Research FoundationEge University [2013/BIL/016]
The authors would like to thank Assoc. Prof. Dr. H. Caglar Karakaya from Izmir Institute of Technology for his valuable technical assistance. This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK, grant no. 212T018) and the Ege University Research Foundation (grant no. 2013/BIL/016).
Databáze: OpenAIRE
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