In vitro biocompatibility of denture relining materials

Autor: Gregory L. Polyzois, Jon E. Dahl, Mary J. Frangou-Polyzois
Rok vydání: 2006
Předmět:
Zdroj: Gerodontology. 23(1)
ISSN: 0734-0664
Popis: Gerodontology 2006; 23; 17–22In vitro biocompatibility of denture relining materialsObjective: The aim of the present study was to evaluate the in vitro biocompatibility of denture reliningmaterials using cell culture tests and a test for irritation mechanisms.Background: Denture relining materials contain non-reacted constituents that may leach out during useinducing local toxic or irritative effects.Materials and methods: One chemically cured, four visible light cured and five dual-cured productswere included. Cured test specimens were used for the filter diffusion test, and extracts of cured specimenswere applied in the MTT and the irritation test using the hen’s egg test-chorioallantoic membrane (HET-CAM) method.Results: Five of the tested materials were slightly or moderately cytotoxic in the filter diffusion test, andone product coated with a liner induced severe toxicity. Cell cultures incubated for 24 hour with the testsamples were more damaged than those incubated for 2 hour. In the MTT test, extracts of nine of the 11products induced cytotoxicity. No extracts showed irritation, whereas the coating and two bonding agentstested were strong irritants.Conclusion: Most of the tested materials contained water soluble, toxic substances that leach out of theproducts and that some time was needed to obtain cytotoxic amounts of the leachables. Many dentalmaterials elicit cytotoxic response, but this does not necessarily reflect the long-term risk for adverse effectsas the oral mucosa is generally more resistant to toxic substances than a cell culture.Keywords: cell culture, dental materials, irritation, resin, prosthesis.Accepted 7 November 2005
Databáze: OpenAIRE
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