Effect of δ-Tocopherol on Mice Adipose Tissues and Mice Adipocytes Induced Inflammation
| Autor: | Yoshimi Yabe, Tomoki Nojima, Chie Takahashi-Muto, Haruka Takeuchi, Mana Nagase, Rieko Tanaka-Yachi, Chikako Kiyose |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_specialty General Chemical Engineering medicine.medical_treatment Interleukin-1beta alpha-Tocopherol Anti-Inflammatory Agents Gene Expression Nitric Oxide Synthase Type II Tocopherols Adipose tissue Inflammation Diet High-Fat Proinflammatory cytokine Mice Dietary Sucrose In vivo 3T3-L1 Cells Internal medicine Gene expression Adipocytes medicine Animals heterocyclic compounds Tocopherol Interleukin-6 Chemistry Vitamin E Body Weight food and beverages General Medicine General Chemistry In vitro Mice Inbred C57BL RAW 264.7 Cells Endocrinology Adipose Tissue lipids (amino acids peptides and proteins) Anti-Obesity Agents Inflammation Mediators medicine.symptom |
| Zdroj: | Journal of Oleo Science. 70:1307-1315 |
| ISSN: | 1347-3352 1345-8957 |
| Popis: | The study aim was to evaluate the potential anti-inflammatory effects of vitamin E analogs, especially α-tocopherol and δ-tocopherol. We used male C57BL/6JJcl mice, which were divided into four groups: the control (C), high-fat and high-sucrose diet (H), high-fat and high-sucrose diet+α-tocopherol (Ha) and high-fat and high-sucrose diet+δ-tocopherol (Hd) groups. The mice were fed for 16 weeks. To the high-fat and high-sucrose diet, 800 mg/kg of α-tocopherol or δ-tocopherol was added more. The final body weight was significantly higher in the H group than in the C group. On the other hand, the final body weight was drastically lower in the Ha group and Hd group than in the H group. However, the energy intake was not significantly different among all groups. Therefore, we assumed that α-tocopherol and δ-tocopherol have potential anti-obesity effect. Besides, inflammatory cytokine gene expression was significantly higher in the epididymal fat of the H group than in the C group. These results showed that inflammation was induced by epididymal fat of mice fed a high-fat and high-sucrose diet for 16 weeks. Unfortunately, addition of α-tocopherol or δ-tocopherol to the diet did not restrain inflammation of epididymal fat. Investigation of the anti-inflammatory effects of α-tocopherol or δ-tocopherol in co-cultured 3T3-L1 cells and RAW264.7 cells showed that δ-tocopherol inhibited increased gene expression of the inflammatory cytokines, IL-1β, IL-6, and iNOS. These results suggest that an anti-inflammatory effect in the δ-tocopherol is stronger than that in the α-tocopherol in vitro. We intend to perform an experiment by in vivo sequentially in the future. |
| Databáze: | OpenAIRE |
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