Clades of huge phage from across Earth’s ecosystems
| Autor: | Lesley A. Warren, Jennifer A. Doudna, Keith Bouma-Gregson, Rohan Sachdeva, Anne-Catherine Lehours, Christine He, Jamie H. D. Cate, Jinglie Zhou, Ray Keren, Alex D. Thomas, Fred R. Ward, Patrick Munk, Joanne M. Santini, Mary E. Power, Susan T.L. Harrison, Cindy J. Castelle, Alexander J. Probst, Raphaël Méheust, Christine L. Sun, Kari M. Finstad, Adi Lavy, Kelly C. Wrighton, Mikayla A. Borton, Lin-Xing Chen, Jillian F. Banfield, Karthik Anantharaman, Yuki Amano, Brandon Brooks, Ibrahim F. Farag, Daniela S. Aliaga Goltsman, David A. Relman, Ronald Amundson, Alexander L. Jaffe, Paula Matheus-Carnevali, Matthew R. Olm, Wen-Jun Li, Shufei Lei, Audra E. Devoto, Susannah G. Tringe, Tara Colenbrander Nelson, Basem Al-Shayeb, Michael J. Morowitz, Rose S. Kantor |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
TRNA modification 030306 microbiology viruses Translation (biology) 15. Life on land Biology Genome 03 medical and health sciences chemistry.chemical_compound Eukaryotic translation chemistry Ribosomal protein Evolutionary biology Transfer RNA Gene DNA 030304 developmental biology |
| DOI: | 10.1101/572362 |
| Popis: | Phage typically have small genomes and depend on their bacterial hosts for replication. DNA sequenced from many diverse ecosystems revealed hundreds of huge phage genomes, between 200 kbp and 716 kbp in length. Thirty-four genomes were manually curated to completion, including the largest phage genomes yet reported. Expanded genetic repertoires include diverse and new CRISPR-Cas systems, tRNAs, tRNA synthetases, tRNA modification enzymes, translation initiation and elongation factors, and ribosomal proteins. Phage CRISPR-Cas systems have the capacity to silence host transcription factors and translational genes, potentially as part of a larger interaction network that intercepts translation to redirect biosynthesis to phage-encoded functions. In addition, some phage may repurpose bacterial CRISPR-Cas systems to eliminate competing phage. We phylogenetically define major clades of huge phage from human and other animal microbiomes, oceans, lakes, sediments, soils and the built environment. We conclude that their large gene inventories reflect a conserved biological strategy, observed over a broad bacterial host range and across Earth’s ecosystems. |
| Databáze: | OpenAIRE |
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