Expression of SERPINA3s in cattle: focus on bovSERPINA3-7 reveals specific involvement in skeletal muscle

Autor: Laure Bremaud, Xavier Blanchet, Arnaud Delavaud, Patrick Pélissier, Brigitte Picard, Didier Delourme, Abderrahman Maftah, Antoine Péré-Brissaud, Lionel Forestier, Nathalie Duprat
Přispěvatelé: Unité de Génétique Moléculaire Animale (UMR GMA), Institut National de la Recherche Agronomique (INRA)-Université de Limoges (UNILIM), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Conseil régional Limousin, Université de Limoges (UNILIM)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Male
bovin
Glycosylation
[SDV]Life Sciences [q-bio]
Sarcomere
Mass Spectrometry
[SHS]Humanities and Social Sciences
Connectin
lcsh:QH301-705.5
Research Articles
Regulation of gene expression
Myomesin
0303 health sciences
biology
General Neuroscience
bovine
030302 biochemistry & molecular biology
α1-antichymotrypsin
serpin
Creatine Kinase
MM Form
muscle squelettique
Recombinant Proteins
race charolaise
medicine.anatomical_structure
Titin
Protein Binding
Immunoprecipitation
Immunology
Blotting
Western
Molecular Sequence Data
antichymotrypsine
Serpin
Real-Time Polymerase Chain Reaction
General Biochemistry
Genetics and Molecular Biology
protease inhibitor
03 medical and health sciences
medicine
Animals
[INFO]Computer Science [cs]
Amino Acid Sequence
skeletal muscle
Muscle
Skeletal
Serpins
030304 developmental biology
alpha(1)-antichymotrypsin
Research
Skeletal muscle
Molecular biology
lcsh:Biology (General)
Gene Expression Regulation
Microscopy
Fluorescence
serpine
expression pattern
Protein Biosynthesis
biology.protein
Creatine kinase
Cattle
Sequence Alignment
Zdroj: Open Biology
Open biology (5), 1-15. (2015)
Open Biology, Vol 5, Iss 9 (2015)
Open Biology, Royal Society, 2015, 5, pp.1-15. ⟨10.1098/rsob.150071⟩
ISSN: 2046-2441
DOI: 10.1098/rsob.150071⟩
Popis: α 1 -Antichymotrypsin is encoded by the unique SERPINA3 gene in humans, while it is encoded by a cluster of eight closely related genes in cattle. BovSERPINA3 proteins present a high degree of similarity and significant divergences in the reactive centre loop (RCL) domains which are responsible for the antiprotease activity. In this study, we analysed their expression patterns in a range of cattle tissues. Even if their expression is ubiquitous, we showed that the expression levels of each serpin vary in different tissues of 15-month-old Charolais bulls. Our results led us to focus on bovSERPINA3-7, one of the two most divergent members of the bovSERPINA3 family. Expression analyses showed that bovSERPINA3-7 protein presents different tissue-specific patterns with diverse degrees of N -glycosylation. Using a specific antibody raised against bovSERPINA3-7, Western blot analysis revealed a specific 96 kDa band in skeletal muscle. BovSERPINA3-7 immunoprecipitation and mass spectrometry revealed that this 96 kDa band corresponds to a complex of bovSERPINA3-7 and creatine kinase M-type. Finally, we reported that the bovSERPINA3-7 protein is present in slow-twitch skeletal myofibres. Precisely, bovSERPINA3-7 specifically colocalized with myomesin at the M-band region of sarcomeres where it could interact with other components such as creatine kinase M-type. This study opens new prospects on the bovSERPINA3-7 function in skeletal muscle and promotes opportunities for further understanding of the physiological role(s) of serpins.
Databáze: OpenAIRE
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